Protective Effect of Hydroethanolic extract of Datura metel leaves against Nicotine induced toxicity in albino rats


  • Gbenga Oluyemi Afolayan Department of Pharmacology, Therapeutics & Toxicology, Faculty of Basic Medical Sciences, University of Lagos, Nigeria.
  • Ifedolapo Adesola Adejumo Department of Pharmacology, Therapeutics & Toxicology, Faculty of Basic Medical Sciences, University of Lagos, Nigeria.
  • Kehinde Sola Salako Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Lagos, Nigeria.
  • Ayomide Mautin Akwe Department of Pharmacology, Therapeutics & Toxicology, Faculty of Basic Medical Sciences, University of Lagos, Nigeria.


Datura metel, Nicotine, Poison,, Nicotine-induced toxicity, Vitamin E


Background: Datura metel (D.metel) is a plant with both poisonous and medicinal properties that have been proven to have great pharmacological potential. It has a wide range of traditional applications and uses in folklore medicine to treat various ailments. Conspicuous marketing of new nicotine-containing formulations has caused an upsurge of nicotine toxicity which is increasingly germane to public health . Fatal overdose of nicotine could occur as a result of suicidal intent or by accident. The current review focuses on the protective effffect of hydroethanolic extract of Datura metel leaves against nicotine-induced toxicity in albino rats.

Methods: For a period of 7-days, nicotine (1mg/kg/day, i.p) was administered to fifive groups of rats in addition to hydroethanolic extract of D.metel leaves at doses of 250, 500, 1000 mg/kg and vitamin E at 50mg/kg/day, p.o . On the 8 th day, the animals were sacrifificed, and organs (kidney, heart, liver) were taken for histopathological analysis. The phytochemical analysis and acute toxicity studies were also determined.

Results: Phytochemical analysis revealed the presence of alkaloid, saponin, phenol, flflavonoid, anthraquinone and steroid. No observable signs of delayed toxicity and mortality for 14 days post treatment up to a maximum dose of 8g/kg/p.o. The intraperitoneal LD50 value was estimated as 3076.1mg/kg/i.p. Post-treatment with D. metel ameliorated the nicotine induced pathomorphological changes .

Conclusion:The dose related attenuation of pathomorphological changes is suggestive of D.metel's protective ability. A basis for further exploration of the potential of D.metel as a possible therapeutic agent in human nicotine induced toxicity can be deduced.


Jepsen, F., & Ryan, M. (2005). Poisoning in children. Current Paediatrics, 15(7), 563-568.

Malangu, N. (2011). Doctoral dissertation, University of Limpopo (Medunsa Campus) Acute Poisoning in Three African Countries: Botswana,

South Africa and Uganda

Lentini, P., Zanoli, L., Granata, A., Signorelli, S. S., Castellino, P., & Dell'Aquila, R. (2017). Kidney and heavy metals-The role of environmental exposure. Molecular medicine reports, 15(5), 3413-3419.

El-Maddah, E. I. (2012). Pattern of Acute Poisoning in Adult Patients Admitted to Tanta Poison Centre-Egypt. The Egyptian Journal of Forensic

Sciences and Applied Toxicology, 220(1245), 1-15.

Aki, E. S., & Alessai, G. S. (2019). General approach to poisoned patient. Poisoning in the Modern World - New Tricks for an Old Dog.

Gooch, M. D. (2017). Toxicologic Emergencies. Patient Transport-E-Book: Principles and Practice, 415.

Idrees, M. I. T. (2016). Doctoral dissertation, University of Gezira. Determination of Nicotine in Different Brands of Cigarettes Using High–Performance Liquid Chromatography.

Hoffmann, D., Hoffmann, I., & El-Bayoumy, K. (2001). The less harmful cigarette: a controversial issue. A tribute to Ernst L. Wynder. Chemical research intoxicology, 14(7) , 767 - 790.

Alkam, T., & Nabeshima, T. (2019). Molecular mechanisms fornicotine iintoxication. Neurochemistry International, 125, 117-126.

Kyriakos, C. N., Filippidis, F. T., Hitchman, S., Girvalaki, C., Tzavara, C., Demjén, T., ... & Vardavas, C. I. (2018). Characteristics and correlates of

electronic cigarette product attributes and undesirable events during e-cigarette use in six countries of the EUREST-PLUS ITC Europe Surveys. Tobacco Induced Diseases, 16. https://doi: 10.18332/Ɵd/93545

Schep, L. J., Slaughter, R. J., & Beasley, D. M. G. (2009). Nicotinic plant poisoning. Clinical Toxicology, 47(8), 771- 781.

Pesta, D. H., Angadi, S. S., Burtscher, M., & Roberts, C. K. (2013). The effects of caffeine, nicotine, ethanol, and tetrahydrocannabinol on exercise performance. Nutrition & metabolism, 10(1), 1-15.

Abreu-Villaça, Y., Seidler, F. J., Qiao, D., Tate, C. A., Cousins, M. M., Thillai, I., & Slotkin, T. A. (2003). Short-term adolescent nicotine exposure has immediate and persistent effects on cholinergic systems: critical periods, patterns of exposure, dose thresholds. Neuropsychopharmacology, 28(11), 1935-1949.

Appiah-Ankam, J., & Hunter, J. M. (2004). Pharmacology of neuromuscular blocking drugs. Continuing Education in Anaesthesia Critical Care & P a i n , 4 ( 1 ) , 2 - 7 .

Smolinske, S. C., Spoerke, D. G., Spiller, S. K., Wruk, K. M., Kulig, K., & Rumackt, B. H. (1988). Cigarette and nicotine chewing gum toxicity in

children. Human toxicology, 7(1), 27-31.https://doi/abs/10.1177/096032718800700105

Tafuri, J., & Roberts, J. (1987). Organophosphate poisoning. Annals of emergency medicine, 16(2), 193-202. X

Eyer P. (2003). The role of oximes in the management of organophosphorus pesticide poisoning. Toxicological reviews, 22(3), 165–190.

Passos ID, Mironidou-Tzouveleki M. (2016) Hallucinogenic plants in the Mediterranean countries. Neuropathology of drug addictions and substance in misuse. Academic Press. (pp. 761 - 772) 6

Bhat, S. D., Ashok, B. K., & Acharya, R. (2012). Exploring the concept of vacha (acorus calamus linn.) Shodhana in Ayurveda. International Journal of Research in Ayurveda & Pha rma cy, 3(3).

Freye, E. (2009). Toxicity of Datura stramonium. In Pharmacology and Abuse of Cocaine , Amphetamines, Ecstasy and Related Designer Drugs ). Springer, Dordrecht. (pp. 217 - 218).https://doi:10.1007/978-90-481-2448-0_34

Al-Snafi AE. Medical importance of Datura fastuosa (syn: Datura metel) and Datura stramoniumA review. IOSR Journal of Pharmacy. 017;7(2):43-58.

Ramachandran A, Venkataraman NS. (2016). Allelopathic effects of aqueous leaf extracts of Datura metel L. on Parthenium hysterophorus L. Lifesciences Leaflets . ; 72: 14 - 22. http.//

Michelson (2008). "Datura ingestion: a review of literature and case reports". Journal of Pharmacology and Toxicological Studies. 25 (7): 198–209.

Satyajit Sarker DD, Gray AI. (2010). Humana Press Inc; 2006 National Research Council. Guide for the care and use of laboratory animals. Natural Products Isolation 2nded. UK.

National Research Council. (2010). Guide for the care and use of laboratory animals.Guide for the care and use of laboratory animals.8th edition.National Academic Press.

Miller LC, Tainter ML (1944). Estimation of LD50 and its error by means of log-probit graph paper. Proc Soc Exp Biol Med. 57:261

Adeneye, A. A., & Adeyemi, O. O. (2008). Hypoglycaemic effects of the aqueous seed extract of Hunteria umbellata in normoglycemic and glucose and nicotine induced hyperglycaemic rats. Planta Medica, 74(09), PA245.

Das, S., Gautam, N., Dey, S. K., Maiti, T., & Roy, S. (2009). Oxidative stress in the brain of nicotineinduced toxicity: protective role of Andrographis paniculata Nees and vitamin E. Applied Physiology, Nutrition, and Metabolism, 34(2), 124-135.

Siqueira, L. M., Ryan, S. A., Gonzalez, P. K., Patrick, S. W., Quigley, J., & Walker, L. R. (2017). Nicotine and tobacco as substances of abuse in

children and adolescents. Pediatrics, 139(1).

Mazurov, A., Hauser, T., & Miller, C. H. (2006). Selective α7 nicotinic acetylcholine receptor ligands. Current medicinal chemistry, 13(13), 1567-1584.

Davidson-Hunt, I. (2000). Ecologi c a l ethnobotany: stumbling toward new practices and paradigms. MASA J, 16(1), 1-3. info/biblioteca/Davidson-Hunt2000Etnobotanica.pdf

Krenzelok, E. P. (2010). Aspects of Datura poisoning and treatment. Clinical Toxicology, 48(2), 104 - 110.

Ganesh S, Radha R and Jayshree N.(2015). A review on phytochemical and pharmacological status of Datura fastuosa linn. International Journal of Multidisciplinary Research and Development; 2(4): 602 - 605.

Akharaiyi FC(2011). Antibacterial, Phytochemical and Antioxidant activities of Datura metel. Int. J. PharmTech. Res. 3(1):478-483.

Ratan L , Meenu B, Anju D and Arun N. (2011). Morpo- anatomical and physicochemical studies of dried seeds of Datura fastuosa Linn. . IRJP; 2 (3): 208-212. https://doi: 10.9790/3013-0702014358

Imo, C., Arowora, K. A., Ezeonu, C. S., Yakubu, O. E., Nwokwu, C. D., Azubuike, N. C., & Sallah, Y. G. (2019). Effects of ethanolic extracts of leaf, seed and fruit of Datura metel L. on kidney function of male albino rats. Journal of Traditional and Complementary Medicine, 9(4) , 271 - 27 .

Barile, F. A. (2019). Anticholinergic and Neuroleptic Drugs. In Barile's Clinical Toxicology (pp. 251-269). CRC Press.

Sanni, M., Ejembi, D. O., Abbah, O. C., Olajide, J. E., Ayeni, G., Usman, S. S., et al. (2014). Phytochemical screening and studies on the effects of oral administration of palm wine extract of datura stramonium on the activity of tissue enzymes and histopathology of albino rats.World Journal of Pharmaceutical research. Volume 3, Issue 6, 137-172

Bauzidi A, Mahdeb N and Kara N.(2011). Toxicity studies of alkaloids of seeds of Datura stramonium and synthesis alkaloids in male rats. J Med Plants Res; 5(15):342 - 343.

Verma, S. K., Srivastava, A. K., & Gangwar, N. K. (2012). Pathomorphological changes induced by Datura stramonium seed toxicity in rats. Indian Journal of Veterinary pathology.Volume 36;Issue 2.

Wink, M. (2012). Medicinal plants: a source of anti-parasitic secondary metabolites. Molecules, 17(11), 12771-12791.

Shepard, G. (2005). Psychoactive botanicals in ritual, religion and shamanism. Ethnopharmacology: Encyclopedia of life support systems (EOLSS), 2, 128-182.

Kennedy, D. O., & Wightman, E. L. (2011). Herbal extracts and phytochemicals: plant secondary metabolites and the enhancement of human brain function. AdvNutr. 2 ( 1 ) : 3 2 – 5 0 .

Willis, L. M., Shukitt-Hale, B., & Joseph, J. A. (2009). Recent advances in berry supplementation and age-related cognitive decline. Current Opinion in Clinical Nutrition & Metabolic Care, 12(1), 91-94.https://doi: 10.1097/MCO.0b013e32831b9c6e

Zhang, L., Ravipati, A. S., Koyyalamudi, S. R., Jeong, S. C., Reddy, N., Smith, P. T., .et al (2011). Antioxidant and anti-inflammatory activities of

selected medicinal plants containing phenolic and flavonoid compounds. Journal of agricultural and food chemistry, 59(23), 12361 - 12367.

Rao, P. S., Kalva, S., Yerramilli, A., & Mamidi, S. (2011). Free radicals and tissue damage: Role of antioxidants. Free radicals and antioxidants, 1(4), 2-7.




How to Cite

Afolayan, G. O., Adejumo, I. A., Salako, K. S., & Akwe, A. M. (2023). Protective Effect of Hydroethanolic extract of Datura metel leaves against Nicotine induced toxicity in albino rats: The Nigerian Journal of Pharmacy, 57(1), 388–395. Retrieved from

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