Formulation and Evaluation of Antioxidant Topical Cream Using Ethanol Extract of Markhamia Tomentosa Leaves

https://doi.org/10.51412/psnnjp.2022.41

Authors

  • Ibilola Cardoso-Daodu Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy. University of Lagos, PMB 12003, Lagos, Nigeria
  • Ebuka Obi Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy. University of Lagos, PMB 12003, Lagos, Nigeria
  • Margaret Ilomuanya Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy. University of Lagos, PMB 12003, Lagos, Nigeria
  • Chukwuemeka Azubuike Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy. University of Lagos, PMB 12003, Lagos, Nigeria

Keywords:

Photo-aging, skin, Formulation, Markahmia tomentosa, scavenging
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Abstract

Background: Global warming which results from the thinning of the ozone layer has led to an increase in the level of exposure to ultraviolet rays from the sun. Long term exposure to these rays can lead to photo-aging. The aim of this study is to develop an antioxidant cream using ethanol extract of Markhamia tomentosa leaves as an active ingredient and to evaluate its physico-chemical properties as well as free radical scavenging and ferric reducing antioxidant activities.

Methods: The leaves of Markhamia tomentosa was obtained from Oke-Igbo, Ondo state, Nigeria in August 2019. The leaves of Markhamia tomentosa were dried, pulverized and macerated to obtain Markhamia tomentosa ethanol extract. Four water-in-oil emulsion creams containing Markhamia tomentosa ethanol leaf extract were formulated using fusion technique. Physico-chemical properties such as the pH, viscosity, skin irritancy, spreadability and organoleptic tests were evaluated. Subsequently the (1, 1-diphenyl1-2-picrylhydrazyl) DPPH radical scavenging and Ferric reducing antioxidant power assays were also carried out.

Results: All formulations were brown, with a smooth and non-greasy feel on dermal application. They had no characteristic smell and were easily washed off the skin. Formulations had a pH that fell withinthe range of 5.5-7.5 which is safe for dermal application. All formulations had a spreadability above 100 mm2 g -1 and exhibited non-Newtonian properties with a decrease in viscosity at the increase of shear stress emphasizing the ease of spreadability. FMT1 (Markhamia tomentosa ethanol leaf extract cream) had the highest antioxidant activity which was concentration dependent, with a percentage radical scavenging activity of 76.48% and an absorbance of 0.276 in the ferric reducing antioxidant power assay, at wavelengths of 517 and 700nm respectively.

Conclusion: This Markhamia tomentosa ethanol leaf extract cream formulation exhibited strong antioxidant properties and can serve as a valuable archetype that can be developed and translated to a pharmaceutical antioxidant formulation for clinical use.

References

Umar SA, Tasduq SA (2022) Ozone Layer Depletion and Emerging Public Health Concerns - An Update on Epidemiological Perspective of the Ambivalent Effects of Ultraviolet Radiation Exposure Frontiers in Oncology 12 (1): 1-11. https://www.frontiersin.org/articles/10.3389/fonc.2022.866733/full

Ilomuanya MO, Akhimien T, Aghaizu C,Adeyinka O, Ajayi T (2018) Polyherbal Antioxidant Topical Preparation Comprising Ethanol Extract of Tetracarpidium conophorum and Ocimum gratissimum: Formulation and Evaluation Dhaka University Journal of Pharmaceutical Sciences 17 (2): 213–219. http://journal.library.du.ac.bd/index.php?journal =DUJPS&page=article&op=view&path%5B%5D=1818

Rao TJ, Chandira M (2020) Study on AntioxidantActivity of Three Ethanolic Extract Polyherbal Formulation International Journal of Pharmaceutical Research and Health Sciences 8 (3): 3155–3160. https://www.academia.edu/43822675/A_Study_on_Antioxidant_Activity_of_Three_Ethanolic_ Extract_Polyherbal_Formulation

Pratima T, Minakshi N, Satish G (2018) Antioxidant, Antimicrobial and Wound Healing Activity of Salvadora persica Twig Extracts Journal of Complementary Medicine and Alternative Healthcare 7 (4): 1-6. https://juniperpublishers.com/jcmah/pdf/JCMAH.MS.ID.555720.pdf

Ibrahim MB, Sowemimo AA, Venables L, Koorbanally NA, Awolola GV, Sofidiya MO, Odukoya OA, Koekemoer T, van de Venter M (2018) Biological evaluation of phytoconstituents from Markhamia tomentosa ethanolic leaf extract South African Journal of Botany 1 (115): 1–36.

https://www.sciencedirect.com/science/article/pi i/S0254629917311602

Jones A, Acquaviva A, Suktham T, Dennis GR, Shalliker RA, Soliven A (2019) Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay Food Analytical Methods 13(3): 608–616. https://link.springer.com/article/10.1007/s12161- 019-01675-5

Bankole E, Adekunle A, Sowemimo A, Umebese E , Abiodun O, Gbotosho, G (2015) Phytochemical screening and in vivo antimalarial activity of extracts from three medicinal plants used in malaria treatment in Nigeria ParasitologyResearch 115(1): 299–305.

https://pubag.nal.usda.gov/catalog/4778099

Ilomuanya M, Akhimien T, Aghaizu C, Adeyinka O, Ajayi T (2018) Polyherbal Antioxidant Topical Preparation Comprising Ethanol Extract of Tetracarpidium conophorum and Ocimum gratissimum: Formulation and Evaluation Dhaka University Journal of Pharmaceutical Sciences 1 7 ( 2 ) : 2 1 3 – 2 1 9 . http://dx.doi.org/10.3329/dujps.v17i2.39178

Chen MX, Alexander KS, Baki G (2016) Formulation and Evaluation of Antibacterial Creams and Gels Containing Metal Ions for

Topical Application Journal of Pharmaceutics, 2016 (5754349):1 - 1 0 . https://doi.org/10.1155/2016/5754349

Ilomuanya M, Ekerebe Z, Cardoso-Daodu I, SowemimoA. (2020) Formulation and Evaluation of Sunscreen Cream Using Detarium senegalense Oil as Base Tropical Journal of Natural Product Research 4(4): 141 – 145 . https://ijpbs.net abstract.php?article=NTU2Mw

Djiobie TG, Tsatsop TR, Mbam PT, Bama V, Bamseck A, Dongmo SS, Ngassoum M (2018) Multi-Response Optimization in the Formulation of a Topical Cream from Natural Ingredients C o s m e t i c s 5 ( 1 ) : 1 - 1 4 . https://www.mdpi.com/2079-9284/5/1/7/htm

Vijayalakshmi M, Ruckmani K (2016) Ferric reducing anti-oxidant power assay in plant extract Bangladesh Journal of Pharmacology 11(3): 570- 5 7 2 . https://scholar.archive.org/work/ti6fdziubrbwnlii bldry3dbmy

Gani FA, Isnaini N, Maryam S (2020) Formulation and Investigation Antioxidant of O/W Cream Containing Euphorbia hirta L. Herb

Extract. E3S Web of Conferences, 151(01001): 1- 4 . https://doaj.org/article/cb12a309680a4335a8405360128f8cbe

Sinjari B, Pizzicannella J, D'Aurora M, Zappacosta R, Gatta V, Fontana A, Trubiani O, Diomede F (2019) Curcumin/Liposome

Nanotechnology as Delivery Platform for Antiinflammatory Activities via NFkB/ERK/pE K Pathway in Human Dental Pulp Treated With 2-HydroxyEthyl MethAcrylate (HEMA) Frontiers i n P h y s i o l o g y 1 0 ( 6 3 3 ) 1 - 1 1 . https://www.frontiersin.org/articles/10.3389/fphys.2019.00633/full

Muthukumarasamy R , Mohd I ( 2 0 1 6 ) Formulation and evaluation of antioxidant cream containing methanolic extract of Piper betel leaves International Journal of Pharma and Bio Sciences 7 ( 4 ) 3 2 3 - 328.https://ijpbs.net/abstract.php?article=NTU2

Mw

Malik F, Suryani S, Ihsan S, Meilany E, Hamsidi R (2020) Formulation of cream body scrub from ethanol extract of cassava leaves (manihot esculenta) as antioxidant Journal of Vocational Health Studies 4(1) : 21 - 28 . h t t p s : / / e -journal.unair.ac.id/JVHS/article/view/21180

Wikantyasning ER, Da'i M, Santosa AB, Zhelsiana DA (2017) Preparation and Antioxidant Activity of Cosmeceutical Creams Containing Spirulina platensis and Zinc Oxide Nanoparticles Advanced Science Letters 23(12): 12502–12505. https://publons.com/publon/36299890/

Maulidya DA, Kahtan MI, Natalia D, Handini M,Vidiyantoro, A. (2018). Anthelmintic Activity of Ethanol Extract of Polygonum minus Leaves against Ascaridia galli. Journal of Microbiology a n d I n f e c t i o u s D i s e a s e s , 2 3 – 2 6 . https://dergipark.org.tr/en/pub/jmid/issue/34164/ 403142

Kozłowska M, Ścibisz I, Przybył J, Ziarno M, Żbikowska A, Majewska E (2021) Phenolic Contents and Antioxidant Activity of Extracts of

Selected Fresh and Dried Herbal Materials Polish Journal of Food and Nutrition Sciences 71(3): 2 6 9 – 2 7 8 .https://www.academia.edu/65358534/Phenolic_Contents_and_Antioxidant_Activity_of_Extracts_of_Selected_Fresh_and_Dried_Herbal_Materials

Kumar D (2021). Anthelmintic, antimicrobialand antioxidant activity of different part of indian origin salvadora persica. Journal of

Medical Pharmaceutical and Allied Sciences, 10 (3), 2729–2736. https://doi.org/10.22270/jmpas.v10i3.1088

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Published

2022-10-09

How to Cite

Cardoso-Daodu, I. ., Obi, E. ., Ilomuanya, M. ., & Azubuike, C. . (2022). Formulation and Evaluation of Antioxidant Topical Cream Using Ethanol Extract of Markhamia Tomentosa Leaves: https://doi.org/10.51412/psnnjp.2022.41. The Nigerian Journal of Pharmacy, 56(2). Retrieved from https://psnnjp.org/index.php/home/article/view/301