Bioactivity of the ethanol extracts of <i>Flabellaria paniculata</i>, <i>Rhapiostylis beninensis</i> roots and <i>Khaya ivorensis</i> Bark Against Multidrug-Resistance Bacteria

Authors

  • Chabula M. Stephen Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Maiduguri, Borno state, Nigeria.
  • Nwamaka H. Igbokwe Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Lagos.
  • Abel O. Idowu Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Lagos.
  • Chijioke E. Ezeobiora Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Lagos.

DOI:

https://doi.org/10.51412/psnnjp.2024.25

Keywords:

Khaya ivorensis, Flabellaria paniculata, Rhaphiostylis beninensis, multidrug resistance (MDR), phytochemical screening, antibacterial activity
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Abstract

Background: Antimicrobial resistance is a global health problem that has led to the loss of effectiveness of many antimicrobial drugs, thus necessitating the continuous search for alternatives, especially from natural sources. Flabellaria paniculata, Rhapiostylis beninensis, and Khaya ivorensis are plants that have been used in traditional medicine to treat microbial infections, but documented
evaluations of their effectiveness in scientific literature are few

Methods: The roots of Flabellaria paniculata, Rhapiostyl beninensis, and bark of Khaya ivorensis were extracted by cold maceration method in 70% ethanol. The extracts were analyzed for their phytochemical content. Clinically isolated bacteria were screened for their multidrug resistance status. The antimicrobial activity of the individual plant extracts against clinical multidrug resistance (MDR) bacterial isolates consisting of Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia and Escherichia coli strains
was evaluated by the agar-well diffusion technique on Mueller Hinton agar. The solid dilution method was used to assess the MIC and MBC of susceptible strains.

Results: The phytochemical screening showed that the three plant extracts contain terpenoids, steroids, and saponins in similar quantities, while flavonoids were more abundant in K. ivorensis (109mg/100g), F. paniculata (52mg/100g), and R. beninensis (38mg/100g) respectively. Eleven, ten, and two of the MDR strains of bacteria tested were susceptible to the inhibitory activity of the
individual extracts of K. ivorensis, F. paniculata, and R. beninensis respectively in a concentration- dependent manner. The range of MIC for K. ivorensis (1.56-12.5 mg/mL) was lower than for F. paniculata (6.25-50 mg/mL) and R. beninensis (12.5-50 mg/mL).

Conclusion: The ethanol extracts of the studied plants showed antimicrobial activity against testedMDR bacterial strains, providing  evidence to justify their traditional use, and demonstrating their potential in treating bacterial infections and as a future source for newer chemotherapeutic agents. 

Author Biography

Chabula M. Stephen, Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Maiduguri, Borno state, Nigeria.

Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy,
University of Lagos.

References

Ismahene Y (2022) Infectious Diseases, Trade, and Economic Growth: A Panel Analysis of Developed and Developing Countries. Journal of the Knowledge Economy 13: 2547–2583. doi:10.1007/s13132-021-00811-z

Antimicrobial Resistance Collaborators (2022). Articles Global burden of bacterial antimicrobial resistance in 2019 : A systematic analysis. Lancet 399(10325):629-655.. doi:10.1016/S0140- 6736(21)02724-0

Chattopadhyay MK, Chakraborty R, Grossart H peter, Reddy GS, Jagannadham MV (2015) Antibiotic Resistance of Bacteria. BioMed Research International 2-4. doi:http://dx.doi.org/10.1155/2015/501658

Reygaert WC (2018) An overview of the antimicrobial resistance mechanisms of bacteria. AIMS Microbiology 4(3):482-501. doi:10.3934/microbiol.2018.3.482

Hodges N, Hanlon G (2013) Essential Microbiology for Pharmacy and Pharmaceutical Science. 1st Editio. John Wiley & Sons, Ltd.

Seukep JA, Ngadjui B, Kuete V (2015) Antibacterial activities of Fagara macrophylla, Canarium schweinfurthii, Myrianthus arboreus, Dischistocalyx grandifolius and Tragia benthamii against multi - drug resistant Gram - negative bacteria. SpringerPlus 4:567 . doi:10.1186/s40064-015-1375-y

Mujeeb F, Bajpai P, Pathak N (2014 ) Phytochemical Evaluation, Antimicrobial Activity, and Determination of Bioactive Components from Leaves of Aegle marmelos. BioMed Research International 11. doi:http://dx.doi.org/10.1155/2014/497606

Usman H, Osuji JC (2007). Phytochemical and in vitro antimicrobial assay of the leaf extract of Newbouldia laevis. African Journal of Traditional, Complementary and Alternative Medicines 4(4):476-480.

Ofeimun JO, Ayinde AA, Eze GI (2021) Methanol Root Extract of Rhaphiostylis beninensis Planch Ex Benth (Icacinaceae) Reverses Alcohol-induced Changes in Sex Hormones and Semen Quality in Male Rats. East and Central African Journal of Pharmaceutical Sciences 24:50-56.

Ovbokhan J, Paulinaus N, State E, State E (2020) Hepatoprotective and antioxidant effect of Rhaphiostylis beninensis ethanol root extract on Carbon Tetrachloride. Animal Research International 17(2):3781-3789. eISSN: 1597- 3115

Ofeimun J, Mbionwu M (2014) Cytotoxic and growth inhibitory activity of aqueous extracts of root and leaf of Rhaphiostylis beninensis Planch ex Benth and Pyrenacantha staudtii Engl (Icacinaceae). Journal of Pharmacy and Bioresources 11(1):8. doi:10.4314/jpb.v11i1.2

Evuen UF, Okolie NP, Apiamu A (2022) Evaluation of the mineral composition, phytochemical and proximate constituents of three culinary spices in Nigeria : a comparative study. Scientific Reports 12: 1 - 17. doi:10.1038/s41598-022-25204-3

Chaikaew P, Ade O, Hamilton AO, Cli O (2020) Spatial characteristics and economic value of threatened species (Khaya ivorensis). Scientific Reports 10:1-8. doi:10.1038/s41598-020-63145- x

Adedeji GA, Ogunsanwo OY, Eguakun FS, Elufioye TO (2018) Chemical composition and termiticidal activity of Khaya ivorensis stem bark extracts on woods. Maderas Cienc Tecnologia 20(3):315-324. doi:10.4067/S0718- 221X2018005003201

Orwa C, Mutua A, Kindt R , Jamnadass R, Anthony S (2009) Khaya ivorensis African mahogany Agroforestree Database: A Tree Reference and Selection Guide Version 4.0. 2 0 0 9 ; 0 : 1 - 5 http://www.worldagroforestry.org/sites/treedbs/treedatabases.asp 0:1-5.

Agbedahunsi JM, Fakoya FA, Adesanya SA (2004) Studies on the anti-inflammatory and toxic effects of the stem bark of Khaya ivorensis (Meliaceae) on rats. Phytomedicine. 11:504-508. doi:10.1016/j.phymed.2003.07.009

Sofidiya MO, Agufobi L, Akindele AJ, Olowe JA, Familoni OB (2012). Effect of Flabellaria paniculata Cav. Extracts on gastric ulcer in rats. BMC Complementary Medicine and Therapies 12:1-6. doi:10.1186/1472-6882-12-168

Sofidiya MO, Taiwo E, Awolola V, Habila J, Koorbanally NA (2019) Gastroprotective Effect and Chemical Constituents of Flabellaria paniculata (Malpighiaceae). Natural Product C o m m u n i c a t i o n s 1 - 5 . doi:10.1177/1934578X19860342

Yusuf I, Arzai AH, Haruna M, Sharif AA, Getso MI (2014) Detection of multi drug resistant bacteria in major hospitals in Kano, North-West, Nigeria. Brazilian Journal of Microbiology 2014;45(3):791-798

Bai HJ, Geng QF, Jin F, Yang YL (2024) Epidemiologic analysis of antimicrobial resistance in hospital departments in China from 2022 to 2023. Journal of Health, Population and Nutrition 43(1):39. doi:10.1186/s41043-024- 00526-2

Duedu KO, Offei G, Codjoe FS, Donkor ES (2017) Multidrug Resistant Enteric Bacterial Pathogens in a Psychiatric Hospital in Ghana : Implications for Control of Nosocomial Infections. International Journal of Microbiology 1-6. doi: 10.1155/2017/9509087

A Abdulmajeed R, Jiman-Fatani A, EL-Hossary D (2021) The Accuracy of Non-Conventional Methods Compared to Conventional Methods for Diagnosis of Bloodstream Infections. Acta Scientific Microbiology 4(9):69-80. doi:10.31080/asmi.2021.04.0913

Rath S, Padhy RN (2015) Antibacterial efficacy of five medicinal plants against multidrug - resistant enteropathogenic bacteria infecting under - hospitalized children. Journal of Integrative Medicine 13(1):45-57. doi:10.1016/S2095- 4964(15)60154-6

Teh CH, Nazni WA, Nurulhusna AH, Norazah A, Lee HL (2017) Determination of antibacterial activity and minimum inhibitory concentration of larval extract of fly via resazurin-based turbidometric assay. BMC Microbioogy (17):36. doi:10.1186/s12866-017-0936-3

Schumacher A, Vranken T, Malhotra A, Arts JJC, Habibovic P (2018) In vitro antimicrobial susceptibility testing methods: agar dilution to 3D tissue-engineered models. European Journal of Clinical Microbiology & Infectious Diseases 37(2):187-208. doi:10.1007/s10096-017-3089-2

Sharifi-Rad M, Fokou PVT, Sharopov F et al. (2018) Antiulcer Agents: From Plant Extracts to Phytochemicals in Healing Promotion. Molecule 23(7):1751. doi: 10.3390/molecules23071751

Tsana R, Mafo M, Ottou M, LS Sidjui, Nnanga N (2019) Antimicrobial and antioxidant activities of ethanolic stem bark and root extracts of Khaya ivorensis A Chev. (Meliaceae). Journal of Pharmacognosy and Phytochemistry 8(4):1393- 1397

Johnson OO, Adeyemi DK, Ayoola GA, Bhat SG, Madayath H (2022) The phytochemistry and pharmacological potentials of Flabellaria paniculata cav. (Malpighiaceae): A review of an unexplored medicinal plant. International Journal of Pharmacognosy and Life Science 3 ( 1 ) : 3 6 - 4 1 . doi:10.33545/27072827.2021.v2.i2a.48

Lasisi AA, Folarin OM, Dare EO, Akinloye OA, Fisuyi MO (2013) Phytochemical, antibacterial and cytotoxic evaluation of Raphiostylis beninensis [Hook F. ex Planch] stem bark extracts. Healing Herbs Practice and Technology 2(3):489-495

Foudah AI, Alqarni MH, Alam A, Salkini MA, Ross SA, Yusufoglu HS (2022) Phytochemical Screening , In Vitro and In Silico Studies of Volatile Compounds from Petroselinum crispum ( Mill ) Leaves Grown in Saudi Arabia. Molelcules 27 (3):934. doi:https://doi.org/10.3390/molecules27030934

Olajuyigbe O, Ashafa A (2014) Chemical Composition and Antibacterial Activity of Essential Oil of Cosmos bipinnatus Cav. Leaves from South Africa. Iranian Journal of Pharmaceutical Research 13(4):1417-1423.

Oyewale OB, Khadijat A Ajibola M (2020) Antibacterial, Free Radical Scavenging and Anticancer Properties of Essential Oil from Khaya Ivorensis A. Juss Stem Bark. International Journal of Development Research 10(01):33341- 33346

Tungmunnithum D, Thongboonyou A, Pholboon A (2018) Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects : An Overview. Medicines (5): 1 - 16 . doi:10.3390/medicines5030093

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Published

2024-11-04

How to Cite

Stephen, C. M., Igbokwe, N. H., Idowu, A. O., & Ezeobiora, C. E. (2024). Bioactivity of the ethanol extracts of <i>Flabellaria paniculata</i>, <i>Rhapiostylis beninensis</i> roots and <i>Khaya ivorensis</i> Bark Against Multidrug-Resistance Bacteria. The Nigerian Journal of Pharmacy, 58(2), 268–277. https://doi.org/10.51412/psnnjp.2024.25

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