Natural products-characterized moringa oleifera leaves methanolic extract and anti-diabetic properties mechanisms of its fractions in streptozotocin-induced diabetic rats

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Chimaraoke Onyeabo
Paul Ndubuisi Anyiam
Anthony Cemaluk C Egbuonu


Background: The study characterized natural products and investigated the mechanisms of antidiabetic properties in Moringa oleifera leaves methanolic extract in Streptozotocin-induced diabetic male rats.

Methods: the natural bioactive compounds in difffferent fractions were obtained by column chromatography on 60-120 silica gel mesh and assayed using gas chromatography-mass spectrometric (GC-MS) technique. Also, possible anti-diabetic properties mechanisms of the fractions of extracts investigated using standard methods.

Results: Lethality study indicated safety of the extract at 5000mg/kgbwt. The GC- MS analysis of the methanolic extract revealed seven out of eleven common bioactive constituents with reported antidiabetic activity. Results of possible anti-diabetic properties mechanisms based on studied glucose homeostasis-related bio-indicators showed signifificant (P<0.05) increase in serum lipoprotein lipase, whole blood GLUT 1, liver homogenate G-6-P-DH, Intestine homogenate Na+ /K+ -ATPase, Muscle homogenate GLUT 4 and liver homogenate AMP-Kinase activities in rats administered with the drug (glibenclamide) and fractions 3,4,5 compared with the diabetic group. A signifificant (P<0.05) inhibition of serum α-amylase activity in rat administered drug and fractions 3,4,5 was observed when compared with diabetic control and fractions 1 and 2 . Histological outcomes of rats' pancreas corroborated these fifindings.

Conclusion: Thus, characterized natural bioactive constituents of M. oleifera fractions exerted concerted potent anti-diabetic activity in streptozotocin-induced diabetic rats by probable mechanisms involving concerted α-amylase inhibition, AMP-kinase and Lipo-protein lipase activation and glucose transport Proteins (GLUT-1 and GLUT-4) and liver homogenate Glucose-6-phosphate dehydrogenase up-regulation.

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How to Cite
Onyeabo, C. ., Ndubuisi Anyiam, P. ., & Cemaluk C Egbuonu, A. . (2022). Natural products-characterized moringa oleifera leaves methanolic extract and anti-diabetic properties mechanisms of its fractions in streptozotocin-induced diabetic rats. The Nigerian Journal of Pharmacy, 56(1), 18–29 | Retrieved from
Author Biographies

Chimaraoke Onyeabo, Department of Biochemistry,

College of Natural Science, Michael Okpara University of Agriculture, Umudike,

PMB 7267, Umuahia, Abia State, Nigeria.

Paul Ndubuisi Anyiam, Department of Biochemistry,

College of Natural Science, Michael Okpara University of Agriculture, Umudike,

PMB 7267, Umuahia, Abia State, Nigeria

Anthony Cemaluk C Egbuonu, Department of Biochemistry,

College of Natural Science, Michael Okpara University of Agriculture, Umudike,

PMB 7267, Umuahia, Abia State, Nigeria.


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