Physicochemical and Antibacterial Evaluation of the methanol extract of <i>Chrysobalanus icaco</i> L. (Chrysobalanceae) Seed and Fractions
DOI:
https://doi.org/10.51412/psnnjp.2026.017Keywords:
Physiochemical, Chrysobalanus icaco, Antimicrobial, PhytochemicalAbstract
Background: The use of plants as antimicrobial agents has gained popularity recently due to the need to explore safer alternative means of combating bacterial infections. The seeds of C. icaco were evaluated for its physicochemical, phytochemical and antimicrobial properties.
Methodology: The pulverized seed was extracted via cold maceration. Standard methods were used to evaluate the physicochemical properties of the powdered drug and phytochemical content of the crude extract of the seeds of C. icaco. The crude methanol extract was partitioned into dichloromethane and aqueous fractions. The antibacterial evaluation of the crude extract and fractions were done using agar well diffusion method.
Result: The phytochemicals present in the methanol crude were saponins, tannins, flavonoids, reducing sugars, alkaloids, steroid, terpenoids and cardiac glycosides. The showed moisture content value was 6.37±0.26%. The antimicrobial result revealed that DCM fraction showed the highest zone of inhibition against S. aureus, P. aeruginosa, S. typhi, and E. coli at a concentration of 100 mg/mL, 200 mg/mL, 100 mg/mL, 25 mg/mLand 200 mg/mLrespectively when compared to the crude seed extract and aqueous fraction. The MIC of crude extract was 50 mg/ml against each bacteria. The MIC of DCM fraction was between 100 mg/ml and 50 mg/ml against each bacteria. The MIC of aqueous fraction between 200 mg/ml and 50 mg/ml against each bacteria.
Conclusions/Recommendation: This study therefore suggest that the crude extract and dichloromethane fraction of the seed of C. icaco possess antibacterial activities again selected isolates.
References
1. Onilude HA, Kazeem MI and Adu OB (2021) Chrysobalanus i caco: A r evi ew of its phytochemistry and pharmacology. Journal of Integrative Medicine 19(1): 13-19
2. Iwu MM (2019) Handbook of African Medicinal Plants. CRC.Press Boca Baton, pp.11-15
3. Rasyid ZA, Farida A, Daud SH, Wiwin, S, Wijaya, KI and Tangke, AE (2020) Bioactivities of Forest Medicinal Plants on Kutai Ethnic (Indonesia) of Tapak Leman (Hippobroma longiflora (L) G. Don). GSC Biological and Pharmaceutical Sciences, 11:91-98
4. Nojosa ECN, Marques GEC, Dos Anjos da Paz S, Reis, JT, Brandão, CM, do Nascimento, AS, Camara, MBP and Dos Santos DR (2023) Bioactivity and antimicrobial evaluation of extracts from Chrysobalanus icaco L. found in the Amazonian maranhense, Brazil. Revista Gestão e Secretariado (GeSec). Journal of Management and Secretarial Science, 14 (9);15537–15551.
5. Apitikori-Owumi JE, Sonibare MA, Marwe AA and Firdous SM (2024) An Ethnobotanical Survey, Pharmacognostic Profile and Phytochemical Analysis Investigation of Chrysobalanus icaco L. Yuzuncu Yil University Journal of Agricultural Sciences, 34(3): 489-504.
6. Kadiri HE (2019) The modulatory effect of Chrysobalanus icaco seed on cadmium induced hepatoxicity and nephrotoxicity in rats. Nigerian. Journal of Pure and Applied Science, 32(2):3428- 3435.
7. Erhenhi AH, Lemmy EE and Ashibuogwu CC (2016) Spices used in Ubulu-Uku community of Delta state. International Journal of Herbal Medicine, 4(3):45
8. Silva JP, Peres AM, Paixão TP, Silva AS, Baetas AC and Barbosa WL(2017) Antifungal activity of hydroalcoholic extract of Chrysobalanus icaco against oral clinical isolates of Candida Species. PharmacognosyResearch, 9: 96-100.
9. Adomi PO, Nana MJ and Owhe-Ureghe UB (2024) Phytochemical properties and antibacterial effects of Aframomum sceptrum,
Chryosobalanus icaco and Piper guineense seeds. World Journal Of Pharmaceutical and Medical Research, 10(7): 45-49
10. Araújo-Filho HG, Dias JDS, Quintans-Júnior LJ, Santos MRV, White PAS and Barreto RSS (2016) Phytochemical screening and analgesic profile of the lyophilized aqueous extract obtained from Chrysobalanus icaco leaves in experimental protocols. Pharmaceutical Biology, 54:3055-3062.
11. Nojosa, ECN, Marques, GEC, dos Anjos da Paz, S, Reis, JT, Brandão, CM, do Nascimento, AS, Camara, MBP and dos Santos, DR (2023) Bioactivity and antimicrobial evaluation of extracts from Chrysobalanus icaco L. found in the Amazonian maranhense, Brazil. Revista Gestão e Secretariado, 14(9): 15537-15551.
12. Habtamu T, Velmurugan S, Addisu A (2018) A Review on Antimicrobial Activity of Medicinal Plants against Human Pathogens. Journal of Natural Science Research, 8(19):10-19
13. Akindele EO, Ehlers SM and Koop JHE (2019) First empirical study of freshwater microplastics in West Africa using gastropods from Nigeria as bioindicators. Limnologica, 78: 9
14. Dhivya R and Manimegalai K (2013) Preliminary Phytochemical Screening and GC- MS Profiling of Ethanolic Flower Extract of
Calotropis gigantea Linn. (Apocynaceae). Journal of Pharmacognosy and Phytochemistry, 2(3):28-32.
15. Nwankwo LU, Obokare EC (2023). Preliminary Phytochemi c a l, Physi cochemi c a l, and Comparative Antibacterial Evaluation of Methanolic Extracts of Ocimum gratissimum Stem against Methicillin - Resistant Staphylococcus aureus and Proteus vulgaris.
InternationalPharmacyActa, 2023: 6(1):e8
16. Clauss D and Berkeley RCW (1986) Genus Bacillus In Bergey's manual of determinative bacteriology, Sneath P H A Baltimore. MD: Williams Wilkins, 2, 1105
17. Cheesbrough M (2004). District Laboratory Practice in Tropical Countries part 2. Low price editions. Cambridge University press, London. p434
18. Hasan NA and Zulkahar IM (2018) Isolation and identification of bacteria from spoiled fruits. AIP Conference Proceedings, 1: 020073
19. Hugo WB and Russel AD (1992) Pharmaceutical th Microbiology, 5 Edition, Blank Well Scientific Publication, Oxford, London. pp. 258-297
20. African Pharmacopoeia (1986) General Methods for Analysis. OAU/SRTC Scientific Publications Lagos, pp. 137-149.
21. Rezaei F and VanderGheynst JS (2010) Critical Moisture content for microbial growth in dried food-processing residues. Journal of the Science of Food and Agriculture, 90(12): 2000-2005. Doi:10.1002/jsfa.4044
22. Chaudha ri RK and Gir a s e NO (2015) Determination of soluble extractives and physicochemical studies of bark of Sesbania sesban (L) Merr. Journal of Chemical and Pharmaceutical Research, 7(8):657-660.
23. Khameneh B, Eskin NAM, Iranshahy M and Bazzaz BSF (2021) Phytochemicals: A Promising weapon in the Arsenal against Antibiotic –Resistant Bacteria. Antibiotics (Basel), 10(9):1044. Doi:10.3390/antibiotics10091044
24. Huang W, Wang Y, Tian W, Cui X, Tu P, Li J, Shi S and Liu X (2022) Biosynthesis Investigations of Terpenoid, Alkaloid, and Flavonoid Antimicrobial Agents Derived from medicinal plants. Antibiotics (Basel) 11(10):1380. Doi:10.3390/antibiotics11101380
25. Ca stilho RO and Kapl an MAC (2011) Phytochemical study and antimicrobial activity of Chrysobalanus icaco. Chemistry of Natural Compounds, 47:436–437.
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