Evaluation of Antimicrobial Activity of Methanol Extracts of three Selected Lower Plants Against Wound Pathogens
DOI:
https://doi.org/10.51412/psnnjp.2025.27Keywords:
Lower plants, Wound, PathogensAbstract
Background: The resistance of bacteria to conventional antibiotics has been of great concern worldwide. This has prompted the need to explore alternative natural sources, such as plants, for safer, cheaper and more effective therapies. The use of medicinal plants in wound care is a common practice in traditional medicine. Therefore, the methanol extracts of three lower plants (Nephrolepis biserrata (Fam: Nephrolepidaceae), Platycerium stemaria (Fam: Polypodiaceae), and Platycerium angolense (Fam: Polypodiaceae)) were assessed for their antimicrobial activities against some wound pathogens.
Methods: Wound swabs were collected from twenty patients presenting with wounds at the University Health Centre in Ile-Ife, cultured and the bacteria isolated were identified using conventional biochemical tests. Antimicrobial Susceptibility Test (AST) was carried out on Mueller-Hinton agar using the disk-diffusion method. The Minimum Inhibitory Concentrations (MIC) of methanol plants' extracts against the identified bacteria were determined using the broth micro-dilution technique.
Results: Among the 20 bacteria isolated, 55% and 45% were Gram-positive and Gram-negative, respectively. The most common was Micrococcus luteus (25%), then Staphylococcus aureus (20%), Pseudomonas aeruginosa (15%), Bacillus spp. (10%), Enterobacter aerogenes (10%), Proteus mirabilis (10%), Escherichia coli (5%), and Klebsiella pneumoniae (5%). The methanol extract of N. biserrata showed the highest antimicrobial activity. However, all the isolates that were resistant to azithromycin antibiotic were not susceptible to all the extracts of the 3 selected plants.
Conclusion: N. biserrata and Platycerium spp. leaves could be useful in the management of wound infections as they exhibited antimicrobial activity against isolated wound pathogens. The results from this study support the ethnobotanical use of the plants in wound treatment
References
Kujath P and Michelsen A (2008) Wounds–from physiology to wound dressing. Deutsches Ärzteblatt International, 105(13):239-248.
https://doi.org/10.3238/arztebl.2008.0239
Basu S and Shukla V (2013) Complications of wound healing. In: Mani R, Romanelli M, Shukla V (eds). Measurements in Wound Healing:
st Science and Practice. 1 edn, Springer London, 109-144.
Falanga V, Isseroff RR, Soulika AM, Romanelli M, Margolis D, Kapp S, Granick M, Harding K (2022) Chronic wounds. Nature Reviews Disease Primers, 8(1):50 https://doi.org/10.1038/s41572-022-00377-3
Yazarlu O, Iranshahi M, Kashani HRK, Reshadat S, Habtemariam S, Iranshahy M, Hasanpour M (2021) Perspective on the application of medicinal plants and natural products in wound healing: A mechanistic review. Pharmacological Research, 174: 105841.
https://doi.org/10.1016/j.phrs.2021.105841
Puca V, Marulli RZ, Grande R, Vitale I, Niro A, Molinaro G, Prezioso S, Muraro R, Di Giovanni P (2021) Microbial species isolated from infected wounds and antimicrobial resistance analysis: Data emerging from a three-year retrospective study. Antibiotics, 10 (10): 1162. https://doi.org/10.3390/antibiotics10101162
Nishanthy M and Saikumar C (2017) Bacteriological Profile and Antibiotic Susceptibility Patterns of Bacteria Isolated From Wound Swab Samples from Patients Attending a Tertiary Care Hospital. Scholars Journal of Applied Medical Sciences, 5(11C):4512-4516.
doi: 10.36347/sjams.2017.v05i11.040
Sorg H, Tilkorn DJ, Hager S, Hauser J, Mirastschijski U (2017) Skin wound healing: an update on the current knowledge and concepts. European Surgical Research, 58(1-2):81-94. https://doi.org/10.1159/000454919
Ahmad I, Malak HA and Abulreesh HH (2021) Environmental antimicrobial resistance and its drivers: a potential threat to public health. Journal of Global Antimicrobial Resistance, 27:101-111. https://doi.org/10.1016/j.jgar.2021.08.001
Cowan and Steel's Manual for the Identification of Medical Bacteria (1993) Cambridge University Press pp 175 – 178
Clinical and Laboratory Standard Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing. 30th Edition, M100, 2020
Dubale S, Kebebe D, Zeynudin A, Abdissa N, Suleman S (2023) Phytochemical Screening and Antimicrobial Activity Evaluation of Selected
Medicinal Plants in Ethiopia. Journal of Experimental Pharmacology, 15:51–62. https://doi.org/10.2147/JEP.S379805
Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P (2007) Ethnopharmacological approaches to wound healing–exploring
medicinal plants of India. Journal of Ethnopharmacology, 114: 103 – 113. https://doi.org/10.1016/j.jep.2007.08.010
Enoch S and Leaper DJ (2008) Basic science of wound healing. Surgery, 26: 31 – 37. https://doi.org/10.1016/j.mpsur.2007.11.005
Imran H, Ahmad M, Rahman A, Yaqeen Z, Sohail T, Fatima N, Iqbal W, Yaqeen SS (2015) Evaluation of wound healing effects between
Salvadora persica ointment and Solcoseryl jelly in animal model. Pakistan Journal of Pharmaceutical Sciences, 28: 1777–1780.
Li J, Chen J, and Kirsner R (2007) Pathophysiology of acute wound healing. Clinics in Dermatology, 25: 9 – 18.
https://doi.org/10.1016/j.clindermatol.2006.09.007
Pallavali RR, Degati VL, Lomada D, Reddy MC, Durbaka VRP (2017) Isolation and in vitro evaluation of bacteriophages against MDR-
bacterial isolates from septic wound infections. PLoSONE, 12: e0179245. https://doi.org/10.1371/journal.pone.0179245
Anguzu JR and Olila D (2007) Drug sensitivity patterns of bacterial isolates from septic post-operative wounds in a regional referral hospital in Uganda. African Health Sciences, 7: 148–154. https://doi.org/10.5555/afhs.2007.7.3.148
Mehta RL, Kellum JA, Shah SV, Molitoris BA, Ronco C, Warnock DG, Levin A (2007) Acute Kidney Injury Network. Acute Kidney Injury Network: Report of an initiative to improve outcomes in acute kidney injury. Critical Care, 11: R31. https://doi.org/10.1186/cc5713
Agnihotri N, Gupta V and Joshi RM (2004) Aerobic bacterial isolates from burn wound infections and their antibiograms–a five-year
study. Burns, 30: 241 – 243. https://doi.org/10.1016/j.burns.2003.11.010
Puca V, Marulli RZ, Grande R, et al. (2021) Microbial Species Isolated from Infected Wounds and Antimicrobial Resistance Analysis: Data Emerging from a Three-Years Retrospective Study. Antibiotics (Basel), 10(10):1162. https://doi.org/10.3390/antibiotics10101162
Appapalam ST, Muniyan A, Vasanthi Mohan K, Panchamoorthy R (2021) A Study on Isolation, Characterization, and Exploration of Multiantibiotic-Resistant Bacteria in the Wound Site of Diabetic Foot Ulcer Patients. International Journal of Lower Extremity Wounds, 20(1):6-
https://doi.org/10.1177/1534734619884430
Dau AA, Tloba S and Daw MA (2013) Characterization of wound infections among patients injured during the 2011 Libyan conflict. Eastern Mediterranean Health Journal, 19 (4): 356-361
Odedina EA, Eletta EA, Balogun RA, Idowu O (2008) Isolates from wound infections at Federal Medical Centre, Bida. African Journal of Clinical and Experimental Microbiology, 9(2): 26-32
Gadzama GB, Zailani SB, Abubakar D, Bakari AA (2007) Bacterial pathogens associated with wound infections at the University of Maiduguri Teaching Hospital, Maiduguri, Nigeria. Kanem Journal of Medical Sciences, 1(1):26-28
Sims GK, Sommers LE, Konopka A (1986) Degradation of pyridine by Micrococcus luteus isolated from soil. Applied and Environmental Microbiology, 51: 963 – 968. https://doi.org/10.1128/aem.51.5.963-968.1986
Ghosh A, Chaudhary SA, Apurva SR, Tiwari T, Gupta S, Singh AK, Katudia KH, Patel MP, Chikara SK (2013) Whole-genome sequencing of Micrococcus luteus strain Modasa, of Indian origin. Genome Announcements, 1:e0007613. https://doi.org/10.1128/genomeA.00076-13
Mohanrasu K, Premnath N, Siva Prakash G, Sudhakar M, Boobalan T, Arun A (2018) Exploring multi potential uses of marine bacteria:
an integrated approach for PHB production, PAHs and polyethylene biodegradation. Journal of Photochemistry and Photobiology B: Biology,
: 55 – 65. https://doi.org/10.1016/j.jphotobiol.2018.05.014
Ferrari VB, Cesar A, Cayô R, Choueri RB, Okamoto DN, Freitas JG, Favero M, Gales AC, Niero CV, Saia FT, de Vasconcellos SP (2019) Hexadecane biodegradation of high efficiency by bacterial isolates from Santos Basin sediments. Marine Pollution Bulletin, 142:309–314. https://doi.org/10.1016/j.marpolbul.2019.03.050
López L, Pozo C, Rodelas B, Calvo C, Juárez B, Martínez-Toledo MV, González-López J (2005) Identification of bacteria isolated from an oligotrophic lake with pesticide removal capacities. Ecotoxicology, 14:299–312. https://doi.org/10.1007/s10646-003-6367-y
Min KR, Zimmer MN, Rickard AH (2010) Physicochemical parameters influencing coaggregation between the freshwater bacteria Sphingomonas natatoria 2.1 and Micrococcus luteus 2. 13. Biofouling, 26: 931 – 940. https://doi.org/10.1080/08927014.2010.531128
Chiereghin A, Felici S, Gibertoni D, Foschi C, Turello G, Piccirilli G, Gabrielli L, Clerici P, Landini MP, Lazzarotto T (2020). Microbial contamination of medical staff clothing during p atien t care activities : performance of decontamination of domestic versus industrial
laundering procedures. Current Microbiology, 77:1159–1166. https://doi.org/10.1007/s00284-020-01919-2
Khayyira AS, Rosdina AE, Irianti MI, Malik A (2020). Simultaneous profiling and cultivation of the skin microbiome of healthy young adult skin for the development of therapeutic agents. Heliyon, 6: e03700. https://doi.org/10.1016/j.heliyon.2020.e03700
Nguyen AT and Oglesby-Sherrouse AG (2016) Interactions between Pseudomonas aeruginosa and Staphylococcus aureus during co-cultivations and polymicrobialinfections. Applied Microbiology and Biotechnology, 100: 6141–6148. https://doi.org/10.1007/s00253-016-
-3
Cendra MDM and Torrents E (2021) Pseudomonas aeruginosa biofilms and their partners in crime. Biotechnology Advances, 49: 107734. https://doi.org/10.1016/j.biotechadv.2021.107734
Orazi G, Jean-Pierre F and O'Toole GA (2020) Pseudomonas aeruginosa PA14 Enhances the Efficacy of Norfloxacin against Staphylococcus
aureus Newman Bio films. Journal of Bacteriology, 202: e00159 - 20. https://doi.org/10.1128/JB.00159-20
Trizna EY, Yarullina MN, Baidamshina DR, Mironova AV, Akhatova FS, Rozhina EV, Fakhrullin RF, Khabibrakhmanova AM, Kurbangalieva AR, Bogachev MI, Kayumov AR (2020) Bidirectional alterations in antibiotics susceptibility in Staphylococcus aureus-Pseudomonas aeruginosa dual-species biofilm. Scientific Reports, 10: 14849. https://doi.org/10.1038/s41598-020-71834-w
Magalhães AP, Jorge P and Pereira MO (2019) Pseudomonas aeruginosa and Staphylococcus aureus communication in biofilm infections: Insights through network and database construction. Critical Reviews in Microbiology, 45: 712 – 728. https://doi.org/10.1080/1040841X.2019.1700209
Cacioppo JT and Hawkley LC (2003) Social isolation and health, with an emphasis on underlying mechanisms. Perspectives in Biology and Medicine, 46: S39–S52
Young A and McNaught C-E (2011) The physiology of wound healing. Surgery 29: 4 7 5 – 4 7 9 .
https://doi.org/10.1016/j.mpsur.2011.06.011
Halawa E M, Fadel M, Al-Rabia MW, Behairy A, Nouh NA, Abdo M, Olga R, Fericean L, Atwa AM, El-Nablaway M and Abdeen A (2024) Antibiotic action and resistance: updated review of mechanisms, spread, influencing factors, and alternative approaches for combating resistance. Frontiers in Pharmacology, 14:1305294. https://doi.org/10.3389/fphar.2023.1305294
Ayaz M, Subhan F, Ahmed J, Khan AU, Ullah F, Ullah I, Ali G, Syed NI, Hussain S (2015) Sertraline enhances the activity of antimicrobial agents against pathogens of clinical relevance. Journal of Biological Research – Thessaloniki, 22: 4. https://doi.org/10.1186/s40709-015-0028-1
Chiou C, Hong Y, Wang Y, Chen B, Teng R, Song H, Liao Y (2023) Antimicrobial Resistance and Mechanisms of Azithromycin Resistance in
Nontyphoidal Salmonella Isolates in Taiwan, 2017 to 2018. Microbiology Spectrum, 11: e03364 - 22. https://doi.org/10.1128/spectrum.03364-22
Ibiye A, Green BO, Ajuru MG, Chikere LC (2024) Phytochemical and proximate compositions of frond extracts of Nephrolepis biserrata, Phymatosorus scolopendria, and Microgramma mauritiana in Rivers State University, Nigeria. Asian Journal of Natural Product Biochemistry,
(1): 1 - 7. https://doi.org/10.13057/biofar/f220101
Bode SO and Oyedapo OO (2010) Biological activities and phytoconstituents of the lower plant Platycerium angolense, Welwex Hook. Journal of Medicinal Plants Research, 5(8): 1321-1329
Yan Y, Li X, Zhang C, Lv L, Gao B, Li M (2021) Research Progress on Antibacterial Activities and Mechanisms of Natural Alkaloids: A Review.
Antibiotics (Basel, Switzerland), 10(3): 318. https://doi.org/10.3390/antibiotics10030318
Shamsudin NF, Ahmed QU, Mahmood S, Ali Shah SA, Khatib A, Mukhtar S, Alsharif MA, Parveen H, Zakaria ZA (2022) Antibacterial
Effects of Flavonoids and Their Structure-Activity Relationship Study: A Comparative Interpretation. Molecules (Basel, Switzerland), 27 (4): 1149.
https://doi.org/10.3390/molecules27041149
Li J and Viviana M (2023) In Vitro and In Silico Studies of Antimicrobial Saponins: A Review. Processes, 11 (10): 2856.
https://doi.org/10.3390/pr11102856
Czerkas K, Olchowik-Grabarek E, Łomanowska M, Abdulladjanova N, Sękowski S (2024) Antibacterial Activity of Plant Polyphenols Belonging to the Tannins against Streptococcus mutans—Potential against Dental Caries. Molecules, 29: 879. https://doi.org/10.3390/molecules29040879
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