Ameliorating Effects of Honey on Ethanol, Caffeine, Morphine and Scopolamine- Novelty Induced Behaviors and Memory Impairment in Male Albino Mice

Authors

  • Ayodele Oluwasoji Akanmu Department of Clinical Pharmacology and Therapeutics, College of Medical Sciences, University of Maiduguri, Maiduguri, Nigeria.
  • Olusayo Oluwole Department of Pharmacology, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
  • Moses Olugbenga Atanda Akanmu Department of Pharmacology, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
  • Isaac Oluwole Adeyemi Department of Clinical Pharmacology and Therapeutics, College of Medical Sciences, University of Maiduguri, Maiduguri, Nigeria.
  • Leonard Mela Paul Department of Clinical Pharmacology and Therapeutics, College of Medical Sciences, University of Maiduguri, Maiduguri, Nigeria.
  • Sulayman Tunde Balogun Department of Clinical Pharmacology and Therapeutics, College of Medical Sciences, University of Maiduguri, Maiduguri, Nigeria.
  • Olufunke Adebola Sodipo Department of Clinical Pharmacology and Therapeutics, College of Medical Sciences, University of Maiduguri, Maiduguri, Nigeria.

DOI:

https://doi.org/10.51412/psnnjp.2024.32

Keywords:

Honey, ethanol, caffeine, morphine, scopolamine, novelty-induced behaviour
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Abstract

Background: Honey is a natural substance produced by honey bees and was found to be useful to humankind since ancient times. It has medicinal properties and found to possess inhibitory effects on the Central Nervous System (CNS).

Methods: Thus, we evaluated its ameliorating effects of honey on scopolamine, morphine, caffeine and ethanol induced behavioral models: Novelty-Induced Behaviors (NIB), learning and memory impairment in male mice.

Results: The results indicated that honey showed a significant effect on morphine and scopolamine- induced locomotor activity {[morphine: [F (3,19) = 11.736; p = 0.0003) and scopolamine: [ F (3,19) = 29.673; p = 0.0001)]}. Honey significantly reduced ethanol, morphine, scopolamine and increased the caffeine effects on rearing behavior [ethanol: [F (3,19) = 13.724; p = 0.0001); morphine: [ F (3,19) = 18.167; p = 0.0001); scopolamine: [ F (3,19) = 5.523; p =0.008 and caffeine: [F (3,19) = 3.506; p = 0.039)] when compared with control groups. In grooming, honey significantly reduced effect of morphine and increased scopolamine-induced behavior [morphine: F (3,19) = 12.895; p = 0.0002) and scopolamine: [ F (3,19) = 9.465; p = 0.0008)]. Honey produced a significant effect on ethanol and
morphine with spatial working memory in mice [ethanol: [ F (3,19) = 5.236; p = 0.010) and morphine: [ F (3,19) = 10.080; p = 0.0006)]. In elevated plus maze test, honey significantly increased the transfer latency of ethanol: [ F (3,19) = 0.08805; p = 9656); morphine: [F (3,19) = 1.610; p = 0.2265; scopolamine: [ F (3,19) = 0.1695; p = 0.9154) and (Caffeine: [ F (3,19) = 0.1736; p = 0.9127]) on spatial working memory impairment in mice.

Conclusion: In conclusion, honey has significant inhibitory effects on ethanol, morphine, scopolamine and caffeine pharmacological effects on the CNS. 

References

Greenish HG (1999). Materia Medica. 3thEdn. Scientific Publishers, Jodhpur (India) pp:522-523.

Bagde AB, Sawant RS, Bingare SD, Sawai RV and Nikumbh MB (2013) Therapeutic and nutritional values of honey [Madhu]. International Research Journal of Pharmacy 4(3):19-22. https://doi.org/10.7897/2230-8407.04305

Amudha K and Sunil G (2013) Potential benefits of honey in type 2 diabetes mellitus: A review. International Journal of Collaborative Research on Internal Medicine & Public Health 5(4): 199- 216. https://www.iomcworld.org/articles/potential-benefits-of-honey-in-type-2-diabetes-mellitus- areview.pdf

Ahmed R, Khan NA and Waseem M (2017) Phytochemistry and Medicinal Importance of Honey - A Review. Journal of Integrated

Community Health 6(3&4): 31-34. https://journals.indexcopernicus.com/api/file/viewByFileId/213935.pdf

Khan IU, Dubey W and Gupta V (2014) Medicinal Properties of Honey: A Review. International Journal of Pure and Applied Bioscience 2(5):149- 156.

Laichena SF (1998) Antimicrobial Food Additives. 2ndEdn. Springer-Verlag Berlin Heidel berg New York, pp: 7.

Miguel MG, Antunes MD, & Faleiro ML (2017) Honey as a Complementary Medicine. Integrative Medicine Insights 12, 1178633717702869. https://doi.org/10.1177/1178633717702869

Vallianou NG, Gounari P, Skourtis A, Panagos J and Kazazis C (2014) Honey and its Anti- Inflammatory, Anti-Bacterial and Anti-Oxidant

Properties. General Medicine (Los Angel), an open access Journal 2(2): 1-5.DOI: 10.4172/2327-5146.1000132

Wilson JI, George BO and Umukoro GE (2011) Effects of honey on the histology of liver in adult wistar rats. Biology and Medicine 3 (1): 1-5. Doi: 10.15406/mojbm.2017.02.00046.

Purbafranil A, Hashemi SAG, Bayyenat S, Moghaddam HT and Saeidi M (2014) The Benefits of Honey in Holy Quran. International Journal of Paediatrics 2(9):67-72. DOI:10.22038/ijp.2014.3417

Manyi-Loh CE, Anna M, Clarke AM and Ndip RN (2011) An overview of honey: Therapeutic properties and contribution in nutrition and human health. African Journal of Microbiology Research 5(8):844-852. http://www.academicjournals.org/ajmr DOI: 10.5897/AJMR10.008

Ali M (2015) Text Book of Pharmacognosy, 2nd Edn. Satish Kumar Jain for CBS,Publishers and distribution Pvt, Limited. Pp. 65-67.

Azman KF, Zakaria R, Othman Z, & Abdul Aziz CB (2018) Neuroprotective effects of Tualang honey against oxidative stress and memory

decline in young and aged rats exposed to noise stress. Journal of Taibah University for Science 12(3), 273–284. https://doi.org/10.1080/16583655.2018.1465275

Kljakic O, Janickova H, Prado VF, Prado MAM 2017) Cholinergic/glutamatergic co- transmission in striatal cholinergic interneurons:

new mechanisms regulating striatal computation. Journal of Neurochemistry 142 Suppl 2, 90–102. https://doi.org/10.1111/jnc.14003

de Leon AS and Tadi P (2022) Biochemistry, Gamma Aminobutyric Acid. [Updated 2022 May 8]. In: StatPearls [Internet]. Treasure Island (FL):

StatPearls Publishing. Qaid EYA, Zakaria R, Mohd Yusof NA, SulaimanSF, Shafin N, Othman Z, Ahmad AH, Abd Aziz CB, Muthuraju S (2020) Tualang Honey Ameliorates Hypoxia-induced Memory Deficits by Reducing Neuronal Damage in the Hippocampus of Adult Male Sprague Dawley Rats. Turkish journal of Pharmaceutical Sciences 17(5):555–564. https://doi.org/10.4274/tjps.galenos.2019.32704

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals. 8th edition. Washington (DC): National Academies Press (US); 2011. Av a i l a b l e f r o m :

https://www.ncbi.nlm.nih.gov/books/NBK54050 / doi: 10.17226/12910

Gomes PB, Noronha EC, de Melo CT, Bezerra JN, Neto MA, Lino CS, Vasconcelos, SM, Viana GS, de Sousa FC (2008) Central effects of isolated fractions from the root of Petiveria alliacea L. (tipi) in mice. Journal of Ethnopharmacology 120(2):209–214. https://doi.org/10.1016/j.jep.2008.08.012

Akanmu MA, Olowookere TA, Atunwa SA, Ibrahim BO, Lamidi OF, Adams PA, Ajimuda BO and Adeyemo LE (2011) Neuropharmacological

effects of Nigerian honey in mice. African Journal of Traditional Complementary Alternative Medicines, 8(3):230‐249.

https://doi.org/10.4314/ajtcam.v8i3.65285

Akanmu AO, Sodipo OA, Sandabe UK, Shamaki BU, Balogun ST and Jubrin J (2021) Novelty- induced behavior and memory enhancing

activities of aqueous and ethanol extracts of Solanum incanum Linn. fruits in mice. African Journal of Pharmacy and Pharmacology 15(2):33-42. https://doi.org/10.5897/AJPP2020.5210

Krebs-Kraft DL, Rauw G, Baker GB, Parent MB (2009) Zero net flux estimates of septal extracellular glucose levels and the effects of

glucose on septal extracellular GABA levels. European Journal of Pharmacology 611(1- 3):44–52. https://doi.org/10.1016/j.ejphar.2009.03.055

Nasri S, Roghani M, Baluchnejadmojarad T, Balvardi M, Rabani T (2012) Chronic cyanidin-3- glucoside administration improves short-term spatial recognition memory but not passive avoidance learning and memory in streptozotocin-diabeticrats. Phytotherapy Research 26(8):1205–1210. https://doi.org/10.1002/ptr.3702

Mamiya T, Asanuma T, Kise M, Ito Y, Mizukuchi A, Aoto H, Ukai M (2004) Effects of pre- germinated brown rice on beta-amyloid protein-

induced learning and memory deficits in mice. Biological and Pharmaceutical Bulletin 27(7):1041–1045.https://doi.org/10.1248/bpb.27.1041

Joshi H and Megeri K (2008) Antiamnesic evaluation of Clerodendrum phlomidis Linn. bark extract in mice. Revista Brasileira de Ciências

Farmacêuticas 44(4):717-725. https://doi.org/10.1590/S1516-93322008000400019

Parle M, Dhingra D, Kulkarni SK (2004) Memory-strengthening activity of Glycyrrhiza glabra in exteroceptive and interoceptive behavioral models. Journal of Medicinal Food 7 ( 4 ) : 4 6 2 – 4 6 6 . https://doi.org/10.1089/jmf.2004.7.462

GraphPad Prism (2007). GraphPad Software Inc., San Diego CA, www.graphpad.com. Ajayi AA and Ukponmwan OE (1994) Possible evidence of angiotensin II and endogenous opioid modulation of novelty-induced rearing in the rat. African Journal of Medicine and Medical Sciences 23(3):287–290. https://pubmed.ncbi.nlm.nih.gov/7604756/

Frye GD, Breese GR (1981) An evaluation of the locomotor stimulating action of ethanol in rats and mice. Psychopharmacology75(4):372–379. https://doi.org/10.1007/BF00435856

Acevedo MB, Nizhnikov ME, Spear NE, Molina JC, Pautassi RM (2013) Ethanol-induced locomotor activity in adolescent rats and the

relationship with ethanol-induced conditioned place preference and conditioned taste aversion. Developmental Psychobiology 55(4):429–442. https://doi.org/10.1002/dev.21048.

Pelloux Y, Costentin J, Duterte-Boucher D (2015) Differential involvement of anxiety and novelty preference levels on oral ethanol consumption in rats. Psychopharmacology 232(15):2711–2721. https://doi.org/10.1007/s00213-015-3910-5

Ziskind-Conhaim L, Gao BX, Hinckley C (2003) Ethanol dual modulatory actions on spontaneous postsynaptic currents in spinal motoneurons. Journal of Neurophysiology 89(2):806–813. https://doi.org/10.1152/jn.00614.2002

Ariwodola OJ, Weiner JL (2004) Ethanol potentiation of GABAergic synaptic transmission may be self-limiting: role of presynaptic GABA(B) receptors. The Journal of Neuroscience24(47):10679–10686. https://doi.org/10.1523/JNEUROSCI.1768- 04.2004

Carta M, Mameli M, Valenzuela CF (2004) Alcohol enhances GABAergic transmission to cerebellar granule cells via an increase in Golgi cell excitability. The Journal of Neuroscience 24(15):3746–3751. https://doi.org/10.1523/JNEUROSCI.0067-04.2004.

Roberto M, Gilpin NW, Siggins GR (2012). The central amygdala and alcohol: role of γ- aminobutyric acid, glutamate, and neuropeptides. Cold Spring Harbor Perspectives in Medicine 2(12), a012195. https://doi.org/10.1101/cshperspect.a012195

Roberto M, Kirson D, Khom S (2021) The Role of the Central Amygdala in Alcohol Dependence. Cold Spring Harbor Perspectives in Medicine, 11(2):a039339. https://doi.org/10.1101/cshperspect.a039339

Tatarczyńska E, Klodzińska A, Chojnacka- Wójcik E, Palucha A, Gasparini F, Kuhn R, Pilc A (2001) Potential anxiolytic- and antidepressant-

like effects of MPEP, a potent, selective and systemically active mGlu5 receptor antagonist. British Iournal of Pharmacology 132(7):1423–1430. https://doi.org/10.1038/sj.bjp.0703923

Rang H, Dale M, Ritter J and Moore P (2003) Pharmacology. Edinburgh, New York: Churchill Livingston. Matell MS, Berridge KC, Aldridge JW (2006) Dopamine D1 activation shortens the duration of phases in stereotyped grooming sequences. Behavioural Processes, 71(2-3), 241–249. https://doi.org/10.1016/j.beproc.2005.09.008

Taylor JL, Rajbhandari AK, Berridge KC, Aldridge JW (2010) Dopamine receptor modulation of repetitive grooming actions in the rat: potential relevance for Tourette syndrome. Brain Research 1322:92-101. doi: 10.1016/j.brainres.2010.01.052.

Sircar R, Basak AK, Sircar D (2009) Repeated ethanol exposure affects the acquisition of spatial memory in adolescent female rats. Behavioural Brain Research 202(2):225–231. https://doi.org/10.1016/j.bbr.2009.03.036

Sey NYA, Gómez-A A, Madayag AC, Boettiger CA, Robinson DL (2019) Adolescent intermittent ethanol impairs behavioral flexibility in a rat foraging task in adulthood. Behavioural Brain Research, 373, 112085. https://doi.org/10.1016/j.bbr.2019.112085

Shiigi Y, Takahashi M, Kaneto H (1990) Facilitation of memory retrieval by pretest morphine mediated by mu but not delta and kappa opioid receptors. Psychopharmacology 02(3):329–332. https://doi.org/10.1007/BF02244099

Bardo MT, Neisewander JL, Pierce RC (1989) Novelty-induced place preference behavior in rats: effects of opiate and dopaminergic drugs. Pharmacology, Biochemistry and Behavior 32(3):683–689. https://doi.org/10.1016/0091-3057(89)90018-x

Kitanaka J, Kitanaka N, Hall FS, Fujii M, Goto A, Kanda Y, Koizumi A, Kuroiwa H, Mibayashi S, Muranishi Y, Otaki S, Sumikawa M, Tanaka K,

Nishiyama N, Uhl GR and Takemura M (2015) Memory Impairment and Reduced Exploratory Behavior in Mice after Administration of

Systemic Morphine. Journal of Experimental Neuroscience 9: 27–35. https://doi.org/10.4137/jen.s25057

Pothos E, Rada P, Mark GP, Hoebel BG (1991) Dopamine microdialysis in the nucleus accumbens during acute and chronic morphine,

naloxone-precipitated withdrawal and clonidine treatment. Brain Research 566(1-2):348–350. https://doi.org/10.1016/0006-8993(91)91724-f

Poorheidari G, Pratt J and Dehghani N (2002) Effects of low‐dose scopolamine on locomotor activity: No dissociation between cognitive and no n ‐eff ects . Neuros cien ce Res earch Communications 31(3):165 – 174. https://doi.org/10.1002/nrc.10049

Rojas-Carvajal M, Chinchilla-Alvarado J, Brenes JC (2022) Muscarinic regulation of self-grooming behavior and ultrasonic vocalizations in the context of open-field habituation in rats. Behavioural Brain Research 418:113641. https://doi.org/10.1016/j.bbr.2021.113641

Nomura Y, Nishiyama N, Saito H, Matsuki N (1994) Role of cholinergic neurotransmission in the amygdala on performances of passive avoidance learning in mice. Biological & Pharmaceutical Bulletin 17(4):490–494. https://doi.org/10.1248/bpb.17.490

Pelsőczi P, Lévay G (2017) Effect of Scopolamine on Mice Motor Activity, Lick Behavior and Reversal Learning in the IntelliCage. Neurochemical Research 42(12), 3597–3602. https://doi.org/10.1007/s11064-017-2408-4

Alshabi AM, Shaikh I.A, Asdaq SMB (2022) The antiepileptic potential of Vateria indica Linn in experimental animal models: Effect on brain GABA levels and molecular mechanisms. Saudi Journal of Biological Sciences 29(5):3600–3609. https://doi.org/10.1016/j.sjbs.2022.02.059

Britton DR, Indyk E (1990) Central effects of corticotropin releasing factor (CRF): evidence for similar interactions with environmental novelty and with caffeine. Psychopharmacology 101(3):366–370. https://doi.org/10.1007/BF02244055

Marin MT, Zancheta R, Paro AH, Possi AP, Cruz FC, Planeta CS (2011) Comparison of caffeine- induced locomotor activity between adolescent and adult rats. European Journal of Pharmacology 660(2-3), 363–367. https://doi.org/10.1016/j.ejphar.2011.03.052

Fisone G, Borgkvist A and Usiello A (2004). Caffeine as a psychomotor stimulant: mechanism of action. Cellular and Molecular Life Sciences: C S, 61(7 - 8), 857 – 872. https://doi.org/10.1007/s00018-003-3269-3

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Published

2024-11-04

How to Cite

Akanmu, A. O., Oluwole, O., Akanmu, M. O. A., Adeyemi, I. O., Paul, L. M., Balogun, S. T., & Sodipo, O. A. (2024). Ameliorating Effects of Honey on Ethanol, Caffeine, Morphine and Scopolamine- Novelty Induced Behaviors and Memory Impairment in Male Albino Mice. The Nigerian Journal of Pharmacy, 58(2), 412–430. https://doi.org/10.51412/psnnjp.2024.32

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