Identification of alkaloids from the methanol Leaf extract of <I>Acalypha wilkesiana</I> using HPLC/GC-MS

Authors

  • Emmanuel Eimiomodebheki Odion Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, Edo State.
  • Amanda Akpevweoghene Odiete Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, Edo State.
  • Eravweroso, Congrat Odiete Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Benin, Benin City, Edo State.

DOI:

https://doi.org/10.51412/psnnjp.2024.03

Keywords:

Phytochemicals, HPLC, GC-MS, Alkaloids, Acalypha Wilkesiana
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Abstract

Background: Different parts of Acalypha wilkesiana has been reportedly used in the treatment of diverse ailments traditionally. These are due to the presence of phytochemicals such as fatty acids, steroids, flavonoids, glycosides and terpenes. The aim of the study was to identify alkaloids in the leaves of A. wilkesiana which have been linked to some of its traditional usage by High Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry.

Methods: Dried, pulverized leaves of Acalypha wilkesiana were macerated with methanol and the extract obtained was subjected to Gas Chromatography-Mass Spectrometry (GC-MS) and High Performance Liquid Chromatography (HPLC) analyses.

Results: Seven alkaloids were identified by GC-MC technique: 1-Phenyl-3,5,7-trimethyl-4,5,6,7-tetrahydropyrazolo(3,4-B)(1,4)diazepine; 1H-1,2,4-Triazole; Indolizine; Methyl-2-oxo1H,5H,6H,7H-cyclopenta[B]pyridine-3-carboxylate; 3-Methyl-2-henylindole; Furazano [3,4-B]pyrazin-5(4H)-one; 1H-Isoindole-1,3(2H)-dione. Also, the HPLC analysis was able to identify five
classes of alkaloidal compounds; quinolinamine, benzenesulfonamide, allylamine, benzamide and indolizine.

Conclusion:The leaves of A. wilkesiana contain useful alkaloids that may be implicated in some of its traditional uses and as source of important bioactive molecules.


References

s

Oso AA. Acalypha wilkesiana: Prospect as an

Agricultural biocides. World Journal of Advance

Research and Reviews 2020;6(1): 166-172

O n o c h a , Pa tri c i a A k p ome d a y e e t a l.

Antimi c robi a l eva lua tion of Nige ri an

Euphorbiaceae plants 1: Phyllanthus amarus and

Phyllanthus muellerianus leaf extracts. Nigerian

Journal of Natural Products and Medicine

;7:9-12.

Okonwu K, Ahunanya VC. Assessment and

Quantification of some Bioactive Components

and Minerals in the Leaves and Roots of two

Acalypha Species. Nigerian Journal of Botany

; 33(2): 241-251.

Adesina SK, Idowu O, Ogundaini AO, Oladimeji

H, Olugbade TA, Onawunmi GO, Pais M.

Antimicrobial constituents of the leaves of

Acalypha wilkesiana and Aacalypha hispida.

Phytother Res. 2000 Aug;14(5):371-4. doi:

1002/1099-1573(200008)14:5<371::aidptr625>3.0.co;2-f. Erratum in: Phytother Res

Dec;14(8):661..

Büssing A, Stein GM, Herterich-Akinpelu I,

Pfüller U. Apoptosis-associated generation of

reactive oxygen intermediates and release of proinflammatory cytokines in human lymphocytes

and granulocytes by extracts from the seeds of

Acalypha wilkesiana. J Ethnopharmacol. 1999

Sep;66(3):301-9. doi: 10.1016/s0378-

(98)00227-x.

Owoyele BV, Okoye OC, Dolor RO, Oloruntola

OP, Soladoye AO. Analgesic, anti-inflammatory

and antipyretic effects of the ethanol extract of

Acalypha wilkesiana leaves in rats. Niger J

Physiol Sci. 2011 Nov 23;26(1):77-82.

Udobang JA, Nwafor PA, Okokon JE. Analgesic

and antimalarial activities of crude leaf extract and

fr a c ti o n s o f Ac a l y p h a wil k e n si a n a . J

Ethnopharmacol. 2010 Feb 3;127(2):373-8. doi:

1016/j.jep.2009.10.028. Epub 2009 Nov 3. .

Din WM, Chu J, Clarke G, Jin KT, Bradshaw TD,

Fry JR, Wiart C. Antioxidant and cytoprotective

effects of an ethanol extract of Acalypha

wilkesiana var. macafeana from Malaysia. Nat

Prod Commun. 2013 Mar;8(3):375-80. PMID:

Sherifat KO, Itohan AM, Adeola SO, Adeola KM,

Aderemi OL. Anti-fungal activity of Acalypha

wilkesiana: a preliminary study of fungal isolates

of clinical significance. Afr J Infect Dis. 2021 Dec

;16(1):21-30. doi: 10.21010/Ajid.v16i1.4.

Erratum in: Afr J Infect Dis. 2022 Dec

;17(1):74.doi: 10.21010/ajid.v16i1.4

Lim SW, Ting KN, Bradshaw TD, et al. Acalypha

wilkesiana extracts induce apoptosis by causing

single strand and double strand DNA breaks. J

Ethnopharmacol 2011 Nov;138(2):616-623. DOI:

1016/j.jep.2011.10.005.

Mahran, G., Shehata, I., Koheil, M., El-Shabrawy,

A., Al-Ahmady, A. Phytochemical and biologicalstudies on two acalypha species. Zagazig. J Pharm

S c i 1 9 9 3 ; 2 ( 2 ) : 1 6 9 - 1 7 7 . d o i :

21608/zjps.1993.187366

Aml a b u WE, No c k IH, Ka u s h i k NK,

Mohanakrishnan D, Tiwary J, Audu PA, Abubakar

MS, Sahal D. Exploration of antiplasmodial

activity in Acalypha wilkesiana Müller

Argoviensis, 1866 (family: Euphorbiaceae) and

its GC-MS fingerprint. Parasitol Res. 2018

May;117(5):1473-1484. doi: 10.1007/s00436-

-5802-1. Epub 2018 Mar 17.

Oyebode OA, Erukainure OL, Koorbanally NA,

Islam MS. Acalypha Wilkesiana 'Java White':

Identification of Some Bioactive Compounds by

Gc-Ms and Their Effects on Key Enzymes Linked

to Type 2 Diabete. Acta Pharm. 2018 Dec

;68(4):425-439. doi: 10.2478/acph-2018-0037.

Anokwuru CP, Sinisi A, Samie A, TaglialatelaScafati O. Antibacterial and antioxidant

constituents of Acalypha wilkesiana. Nat Prod

R e s . 2 0 1 5 ; 2 9 ( 1 2 ) : 1 1 8 0 - 3 . d o i :

1080/14786419.2014.983105. Epub 2014 Nov

Onyeike EN, Ikewuchi JC, Ikewuchi CC,

Uwakwe AA Quantitative high performance

liquid chromatography analysis of simple

terpenes, carotenoids, phytosterols and flavonoids

in the leaves of Acalypha wilkesiana MuellArg.

Pacific Journal of Science Technology

;11:480–487.

Odion EE, Ogboru RO and Ighene MO.

Identification_of Compounds_in Elaeis

guineensis Fruits using GC-MS. Dhaka

University Journal of Pharmaceutical Sciences

0 2 0 ; 1 9 ( 2 ) : 1 5 3 - 1 5 9

doi.org/10.3329/dujps.v19i2.50631.

Odion EE, Ambe DA, Idiakose GO, Odiete CE

Phytochemical Screening, Proximate Analysis

and Chromatographic Analysis of methanol leaf

extract of Chromolaena odorata (L) M. King and

H. Rob (Asteraceae). West Africa Journal of

Pharmacy 2023;34(2); 1-11.

Osibote EA, Nwafor SP and Iluobe HO. Chemical

and Phytochemical Analyses of Extracts from the

Leaves of Acalypha wilkesiana, “an Herbal Plant

used for the Treatment of Various Skin Disorders”

Annals of Science and Technology 2020; A5(2);

-48.

Igwe KK, Madubuike AJ, Otuokere IE, Chika I,

Amaku FJ (2016) Studies on the Medicinal Plant

Ac a l y p h a wil k e si a n a Et h a n o l Ex tr a c t

Phytocomponents by GC-MS Analysis. Global

Journal of Science Frontier Research: D

Agriculture and Veterinary 2016;16(2: 49-55.

Sulaiman AN, James AE. Phytochemical

Constituents and Antifungal Activity of Acalypha

wilkesiana Leaves on Candida albicans. Fudma

Journal of Sciences 2023;7(3); 209 - 214.

doi.org/10.33003/fjs-2023-0703-1735.

Szkatuła D, Krzyżak E, Stanowska P, Duda M,

Wiatrak B. A New N-Substituted 1H-Isoindole1,3(2H)-Dione Derivative-Synthesis, Structure

and Affinity for Cyclooxygenase Based on In Vitro

Studies and Molecular Docking. Int J Mol Sci.

0 2 1 J u l 1 8 ; 2 2 ( 1 4 ) : 7 6 7 8 . d o i :

3390/ijms22147678..

Sharma V and Kumar V (2014) Indolizine: a

biologically active moiety. Medicinal Chemistry

R e s e a r c h 2 0 1 4 ; 2 3 ( 8 ) : 3 5 9 3 – 3 6 0 6

doi:10.1007/s00044-014-0940-1.

Dyachenko IV, Dyachenko VD, Dorovatovskii

PV, Khrustalev VN, Nenajdenko VG Novel

multicomponent synthesis of 6,7-dihydro-5Hcyclopenta[b]pyridine derivatives. Chemistry of

Heterocyclic Compounds 2020;56(12): 1592-

doi:10.1007/s10593-020-02854-7.

Xie Y, Su N, Yang J, Tan Q, Huang S, Jin M, Ni Z,

Zhang B, Zhang D, Luo F, Chen H, Sun X, Feng

JQ, Qi H, Chen L. FGF/FGFR signaling in health

and disease. Signal Transduct Target Ther. 2020

Sep 2;5(1):181. doi: 10.1038/s41392-020-

-7

de Souza-Melo WO, Figueiredo-Júnior EC, Freire

JCP, Costa BP, Lira AB, Freires IA, Cavalcanti

YW, Lopes WS, Tavares JF, Pessôa HLF, Pereira

JV. Phytochemistry, antifungal and antioxidant

activity, and cytotoxicity of Byrsonima

gardneriana (A. Juss) extract. Arch Oral Biol.

0 2 1 M a r ; 1 2 3 : 1 0 4 9 9 4 . d o i :

1016/j.archoralbio.2020.104994. Epub 2021

Jan 11.

Popiołek Ł. Hydrazide-hydrazones as potential

antimicrobial agents: overview of the literature

since 2010 Medicinal Chemistry Research 2017;

(2):287-301 doi: 10.1007/s00044-016-1756-y.

Zhang X, Tao F, Cui T, Luo C, Zhou Z, Huang Y,

Tan L, Peng W, Wu C. Sources, Transformations,

Syntheses, and Bioactivities of MonoterpenePyridine Alkaloids and Cyclopenta[c]pyridine

D e r i v a ti v e s. M o l e c u l e s. 2 0 2 2 O c t

4 ; 2 7 ( 2 1 ) : 7 1 8 7 . d o i :

3390/molecules27217187

Ram VJ, Sethi A, Nath M, and Pratap R The

Chemistry of Heterocycles Nomenclature and

Chemistry of Three-to-Five Membered

Heterocycles; Chapter 5 - Five-Membered

Heterocycles. 149-478(2019).

Abdellatif KR, Lamie PF, Omar HA. 3-methyl-2-

phenyl-1-substituted-indole derivatives as

indomethacin analogs: design, synthesis and

biological evaluation as potential antiinflammatory and analgesic agents. J Enzyme

Inhib Med Chem. 2016;31(2):318-24. doi:

3109/14756366.2015.1022174. Epub 2015

Mar 23.

Zhang Q, Shen Q, Gao L, Tong L, Li J, Chen Y, Lu

W. Pyrazolo[4,3-b]pyrimido[4,5-e][1,4]diazepine

derivatives as new multi-targeted inhibitors of

Aurora A/B and KDR. Eur J Med Chem. 2018;

(5); 428-441.

Hussein HJ, Hadi MY and Hameed IH. Study of

chemical composition of Foeniculum vulgare

u s i n g F o u r i e r t r a n s f o r m i n f r a r e d

spectrophotometer and gas chromatography -

mass spectrometry. Journal of Pharmacognosy

a n d Ph y t o t h e r a p y 2 0 1 6 ; 8 ( 3 ): 6 0 - 8 9

https://doi.ord/10.5897/JPP2015.0372.

Fidman B, Nogid A. Role of Tapentadol

Immediate Release (Nucynta) in the Management

of Moderate-to-Severe Pain. P T. 2010

Jun;35(6):330–57. PMCID: PMC2888545.

Boligon AA and Athayde ML. Importance of

HPLC in Analysis of Plants Extracts. Austin

Chromatogr. 2014;1(3): 2.

Sarker SD and Nahar L. Applications of High

Performance LiquidChromatography in the

Analysis ofHerbal Products. In Book: Mukherjee

PK (ed). Evidence-Based Validation of Herbal

th Medicine. 2 Edn. India: Elsevier, 2015;405-425

Dowd FJ, Johnson BS and Mariotti AJ. Antifungal

and Antiviral Agents. In: Pharmacology and

Therapeutics for Dentistry: 7th Edn. Mosby;

Elsevier, 2017;488-503

Szekeres T, Sedlak J and Novotny L. Benzamide

riboside, a recent inhibitor of inosine 5'-

monophosphate dehydrogenase induces

transferrin receptors in cancer cells Current

Medicinal Chemistry 2002;9(7):759-64 doi:

2174/0929867024606911

Cosi C, Guerin K, Marien M, Koek W, Rollet K.

The PARP inhibitor benzamide protects against

kainate and NMDAbut not AMPAlesioning of the

mouse striatum in vivo. Brain Res. 2004 Jan

6 ; 9 9 6 ( 1 ) : 1 - 8 . d o i :

0 . 1 0 1 6 /j. b r a i n r e s. 2 0 0 3 . 0 9 . 0 7 2 . d o i:

1016/j.brainres.2003.09.072

Kim WJ, Beardsley DI, Adamson AW, Brown KD.

The monofunctional alkylating agent N-methylN'-nitro-N-nitrosoguanidine triggers apoptosis

through p53-dependent and -independent

pathways. Toxicol Appl Pharmacol. 2005 Jan

;202(1):84-98. doi: 10.1016/j.taap.06.009

Woon EC, Thr e adgill MD. Poly(ADPribose)polymerase inhibition - where now? Curr

Med Chem. 2005;12(20):2373-92. doi:

2174/0929867054864778.

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Published

2024-04-30

How to Cite

Odion, E. E., Odiete, A. A., & Odiete, E. C. (2024). Identification of alkaloids from the methanol Leaf extract of <I>Acalypha wilkesiana</I> using HPLC/GC-MS . The Nigerian Journal of Pharmacy, 58(1), 25–32. https://doi.org/10.51412/psnnjp.2024.03

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Vol. 58 No. 1 (2024): The Nigerian Journal of Pharmacy

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Research Article

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