Phytochemical and Heavy Metal Screening of Fura da Nono, Ciklavit®, Katoka Mixtures®, and Yoyo Bitters®
Keywords:Fura da Nono, Ciklavit®, Katoka Mixtures®, Yoyo Bitters®, Hazard Index
Background: Evaluation of the phytochemicals and heavy metals contained in four commonly consumed beverage and herbal products in Nigeria, was investigated. This was to ascertain the health benefits as well as potential for toxicity that may be associated with the consumption of these products.
Methods: The samples studied are Fura da Nono, Ciklavit®, Katoka Mixtures® and Yoyo Bitters®. The phytochemical screening of each product was done using standard procedures. The heavy metals screening was done using Agilent®720 Inductively Coupled Plasma -Optical Emission Spectroscopy machine (ICP-OES). The Hazard quotient and Index were assessed using appropriate equations in line with United States Environmental ProtectionAgency (USEPA).
Results: All phytochemicals tested were found in Fura da Nono except Steroids and Terpenoids.Also, all the tested phytochemicals were detected in Ciklavit® except Alkaloids and Phlobatannins. Both Katoka Mixtures® and Yoyo Bitters® contained all other phytochemicals except Phlobatannins. Quantitatively, Fura da Nono was high in Cardiac glycosides, and Ciklavit® was found to be high in Flavonoid content. Both Katoka Mixtures® and Yoyo Bitters® are high in Phenols. The results of the heavy metal analysis revealed different concentrations (ppm) of arsenic, mercury, lead, cadmium, zinc, manganese, copper and nickel in the four products. Arsenic was not detected only in Fura da Nono. The heavy metal concentrations obtained were all within WHO maximum permissible limits. Further analysis for potential non-carcinogenic health risk using Hazard quotient on each of the four toxic heavy metals (arsenic, mercury, lead and cadmium) and Hazard Index (HI) on the same toxic heavy metals revealed that by the United States Environmental ProtectionAgency (USEPA) threshold value of HI ≤1, Yoyo Bitters® (0.4684) and Ciklavit® (0.7870) had the lower and safer values for combined heavy metal risk while Katoka Mixtures® (0.9908) and Fura da Nono (1.4144) had the highest.
Conclusion: The phytochemical screening of the investigated products showed that the commonly consumed food drink and herbal products, are rich in phytochemicals. Even though the heavy metal screening indicates a fairly positive safety margin for three of the products. Caution should therefore be exercised in the use of these products.
Bennett, BC (2010) Plants as food In Economic Botany, B. Bennett (Ed.), Encyclopedia of Life Support Systems (EOLSS) Oxford, UK: EOLSS Publishers.
Hexa Research (2017) Global Herbal Medicine Market Size, Value, 2014-2024, Industry Report. h t t p s : / / w w w. h e x a r e s e a r c h . c o m / r e s e a r c h - report/global-herbal-medicine-marketAccessed June 2 2021.
Euromonitor (2021) Herbal/Traditional products i n N i g e r i a . https://www.euromonitor.com/herbaltraditionalproducts-in- nigeria/report#.AccessedApril 19 2022.
Jideani VA, Nkama I, Agbo EB, Jideani IA. (2001). Survey of fura production in some northern states of Nigeria. Plant Foods Hum Nutr. 56(1):23-36. https://doi: 10.1023/a:1008116118051.
Umar A, Yakubu MS, Rabe AM, Yusuf A, and Sumayya BU. (2013). Sensory evaluation of Fura made from malted and unmalted grains of millet. IOSR Journal of Environmental Science, Toxicology and Food Technology 4(4): 86-92.
Abah CR, Ishiwu C, Obiegbuna J, and OladejoAA (2020) Nutritional composition, functional properties and food applications of millet grains. Asian Food Science Journal 14(2): 9-19.
Akinleye MO, Amaeze OU, Opeodu OT, and Okubanjo OO. (2016). Effect of Ciklavit® - a Nigerian poly-herbal formulation on the dissolution profile of proguanil tablets: potential for herb-drug interaction. British Journal of Pharmaceutical Research 12(6): 1-9. https://doi.org/10.9734/BJPR/2016/28298
World Health Organization (2006). Sickle-cell anaemia: report by the Secretariat Fifty Ninth World Health AssemblyA59/9. https://apps.who.int/iris/handle/10665/20890. Accessed June 21 2020
Anionye JC, Onyeneke EC. (2016). Composition and in vitro antioxidant capacity of Yoyo Bitters®. European Journal of Biological Sciences 8(3): 108- 115. http://www.doi:10.5829/idosi.ejbs.2016.108.115
Kale OE, Akinpelu OB, Bakare AA, Yusuf FO, Gomba R, Araka DC, Ogundare TO, Okolie AC, Adebawo O, Odutola O. (2018). Five traditional Nigerian polyherbal remedies protect against high fructose fed, streptozotocininduced type 2 diabetes in male wistar rats. BMC Complementary and Alternative Medicine 18(160): 1-11. https://doi.org/10.1186/s12906-018-2225-6
Kayode AAA, Kayode OT, Adebayo OM. (2019). Therapeutic effect of yo-yo bitters and evans healthy bitters against acetaminophen-induced hepatotoxicity in mice. International Journal of Innovative Science and Research Technology 4(2): 590–594.
Martins OC, Akinleye MO, Ukpo GE, AdepojuBello AA, Odediran BB, Onun EO, Makinde PA. (2018). Impact of Yoyo Bitters® on the pharmacokinetic profile of ciprofloxacin in healthy Nigerian volunteers. Tropical Journal of Natural Product Research 2(10): 456–459. http://www.doi.org/10.26538/tjnpr/v1i4.5
Bussman FW, Glen A, Meyer K, Kuhlman A, Townsmith A. (2010). Herbal mixtures in traditional medicine in Northern Peru Journal of Ethnobiology and E t h n o m e d i c i n e 6 ( 1 0 ) : 1 - 1 1 . https://doi.org/10.1186/1746-4269-6-10.
Omotosho IO, Olusanya T, Onyeaghala AO. (2018). Biochemical studies on toxicity of Yoyo Bitters® (a polyherbal preparation) on liver and kidney functions of wistar rats. Archive of Basic and Applied Medicine 6: 177–182.
Luo L, Wang B, Jiang J, Fitzgerald M, Huang Q, Yu Z, Li H, Zhang J, Wei J, Yang C, Zhang H, Dong L, Chen S. (2021). Heavy metal contaminations in herbal medicines: determination, comprehensive risk assessments, and solutions. Frontiers in Pharmacology 14: 1-14. https://doi.org/10.3389/fphar.2020.595335
Nwachukwu RE, Ihedioha NJ, Ayogu IJ, OgbefiChima IF, Onoja US, Alum LO. (2018). Assessment of heavy metals content of some plant-based medicines in parts of southern Nigeria, West Africa. International Journal of Pharmaceutical Sciences and Research 9(2): 775-783. https://doi.org/10.13040/IJPSR.0975- 8232.9(2).775-83.
Manousi N, Isaakidou E, Zachariadis GA. (2022) An Inductively Coupled Plasma Optical Emission Spectrometric Method for the Determination of Toxic and Nutrient Metals in Spices after Pressure-Assisted D i g e s t i o n . A p p l i e d S c i e n c e s 1 2 : 1 – 1 0 . https://doi.org/10.3390/app12020534.
Meseret M, Ketema G, Kassahun H. (2020). Health risk assessment and determination of some heavy metals in commonly consumed traditional herbal preparations in Northeast Ethiopia. Journal of Chemistry 1- 7. https://doi.org/10.1155/2020/8883837
Harborne, JB. (1973). Methods of plant analysis In Phytochemical Methods London: Chapman and Hall
Brunner J.H (1984) Direct spectrophotometer determination of saponin. Analytical Chemistry 34: 1314- 1326.
T r e a s e G E a n d E v a n s W C . ( 1 9 8 9 ) . Pharmacognosy (11th Edition) London: Bailliere Tindall, 45-50.
Sofowora A. (1993). Medicinal Plants and Traditional Medicine in Africa (2nd Edition) Chichester, West Sussex, United Kingdom: John Wiley and Sons Limited, 96-106.
Adepoju-Bello AA, Okeke CP, Bamgbade and Oguntibeju OO. (2013). Determination of the concentration of selected heavy metals in indigenous plant: Telfairia occidentalis.Alternative and Integrative Medicine 2 ( 7 ) : 1 - 4 . h t t p : / / w w w. d o i : 1 0 . 4 1 7 2 / 2 3 2 7 - 5162.1000137
World Health Organization (2007) WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues Geneva, Switzerland: WHO Press.
United States Environmental Protection Agency (2023) Cadmium; CASRN 7440-43-9 (Chemical Assessment Summary) United States Environmental Protection Agency Integrated Risk Information System ( I R I S ) . https://iris.epa.gov/ChemicalLanding/&substance_n mbr=141 Accessed April 6 2023
Gomah LG, Ngumbu RS, Voegborlo RB (2019) Dietary exposure to heavy metal contaminated rice and health risk to the population of Monrovia Journal of Environmental Science and Public Health 3: 474-482. https://doi.org/10.26502/jesph.96120077.
De-Katoka (W. A.) Ltd. (2019). Katoka Mixtures® for Fever, Malaria, Headache (25 Cl). https://dekatoka.com/shop/katoka-mixtures-forfever-malaria- headache.Accessed May 5 2020.
Abllat Company Nigeria Limited. (2020). Yoyo® Cleanser Bitters [Leaflet] Ikotun: Abllat Company Nigeria Limited.
Bhatla, SC (2018) Secondary metabolites, In Bhatla, SC and Lal, MA (Ed.) Plant Physiology, Development and Metabolism Singapore: Springer Nature, 1099 – 1163. https://doi.org/10.1007/978-981-13- 2023-1.
Imaga NOA, Ogunnusi OT. (2014). Biochemical assessment of the therapeutic properties of Yoyo® herbal bitters. International Journal of Biology, Pharmacy and A l l i e d S c i e n c e s 3 ( 1 ) : 8 - 2 0 . https://doi.org/10.1016/j.hermed.2013.05.003
Morsy N. (2017). Cardiac glycosides in medicinal plants, In Aromatic and medicinal plants– back to nature L o n d o n : I n t e c h o p e n , 2 9 - 4 5 . https://doi.org/10.5772/65963.
Chandrasekara A, Shahidi F. (2010) Content of insoluble bound phenolics in millets and their contribution to antioxidant capacity Journal of Agricultural and Food C h e m i s t r y 5 8 : 6 7 0 6 – 6 7 1 4 . https://doi.org/10.1021/jf100868b.
Călinoiu LF, Vodnar DC (2018) Whole grains and phenolic acids: a review on bioactivity, functionality, health benefits and bioavailability. Nutrients 10(11): 1615, 1-13. https://doi.org/10.3390/nu10111615.
Iweala EEJ, Uhegbu FO, Ogu GN. (2010). Preliminary in vitro antisickling properties of crude juice extracts of Persia Americana, Citrus Sinensis, Carica Papaya and Ciklavit®. African Journal of Traditional, Complementary and Alternative Medicine 7(2): 113–117. https://doi.org/10.4314/ajtcam.v7i2.50867
Imaga N. (2013). Phytomedicines and nutraceuticals: alternative therapeutics for Sickle Cell A n e m i a . T h e S c i e n t i fi c Wo r l d J o u r n a l 1 - 1 2 . https://doi.org/10.1155/2013/269659
Koomson DA, Kwakye BD, Darkwah WK, Odum B,Asante M,Aidoo G. (2018). Phytochemical constituents, total saponins, alkaloids, flavonoids and vitamin C contents of ethanol extracts of five Solanum torvum fruits. P h a r m a c o g n o s y J o u r n a l 1 0 ( 5 ) : 9 4 6 - 9 5 0 . https://doi.org/10.5530/pj.2018.5.160
Vona R, Sposi NM, Mattia L, Gambardella L, Straface E, Pietraforte D. (2021). Sickle cell disease: role of oxidative stress and antioxidant therapy antioxidants A n t i o x i d a n t s 1 0 ( 2 ) : 2 9 6 , 1 - 2 7 . https://doi.org/10.3390/antiox10020296
Abernethy DR, DeStefano AJ, Cecil TL, Zaidi K, Williams RL, and the USPMetal ImpuritiesAdvisory Panel (2010) Metal Impurities in Food and Drugs Pharmaceutical R e s e a r c h 2 7 ( 5 ) : 7 5 0 - 7 5 5 . https://doi.org/10.1007/s11095-010-0080-3
Onwordi CT, Agbo N, Ogunwande IA. (2015). Levels of Potentially Toxic Metals In Selected Herbal Medicines In Lagos, Nigeria. Journal of Natural Sciences Research 5(2): 148-156.
Afieroho OE, Achara F, Adewoyin B, Abo KA. (2018). Determination of cadmium, chromium and lead in four brands of herbal bitters preparation sold in Benin-city, Southern Nigeria. African Journal of Environmental Science and Technology 12(5): 186-190.
Yan A, Wang Y, Tan SN, Mohd YML, Ghosh S, Chen Z. (2020). Phytoremediation: A Promising Approach for Revegetation of Heavy Metal-Polluted Land. Frontiers i n P l a n t S c i e n c e 1 1 : 1 - 1 0 . https://doi.org/10.3389/fpls.2020.00359
Khan A, Khan S, Khan MA, Qamar Z, Waqas M. (2015). The uptake and bioaccumulation of heavy metals by food plants, their effects on plants nutrients, and associated health risk: a review. Environmental Science and Pollution Research International 22(18): 13772-13799. https://doi.org/10.1007/s11356-015-4881-0
Ahmed KS, Mohamed AR. (2005). Heavy metals (Cd, Pb) and trace elements (Cu, Zn) contents in some foodstuffs from the Egyptian market. Emirate Journal of F o o d a n d A g r i c u l t u r e 1 7 : 1 – 8 . https://doi.org/10.9755/ejfa.v12i1.5046
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