Investigation Of Anti-Inflammatory Activity Of Fractions From The Methanol Extracts Of The Leaf Of Tetrapleura Tetraptera (Schumach & Thonn) Taub
Keywords:
Anti-inflammatory, albumin denaturation, fractions, membrane stabilization, Tetrapleura tetrapteraleafAbstract
Background: Tetrapleura tetraptera (Schumach & Thonn) Taub has many folklore uses mainly in the management ofBackground: convulsion, leprosy, inflammation and rheumatic pains, schistosomiasis, asthma and hypertension. This study was focused
at investigating the anti-inflammatory activitys of various fractions from the methanol extract of Tetrapleura tetraptera.
Methods: The methanol extract was obtained by cold maceration, and the fractions were carried out by liquid-liquid
extraction procedure. Anti-inflammatory activities were evaluated using two acute anti-inflammatory models: Inhibition of
albumin denaturation and Membrane stabilization test.
Results: The results indicate that the different solvent fractions (n-hexane, ethylacetate, chloroform, butanol and aqueous)
ofTetrapleura tetrapteraleaf possess varying anti-inflammatory activity; at stabilizing the Red Blood Cells membrane at
concentration of 200 and 1000 µg/ml respectively. The aqueous extract exhibited maximum inhibition (77.84%) at the
concentration of 1000 µg/ml followed by (75.33 %) at 200µg/ml then n-hexane fraction (70.58 %) at 1000µg/ml while the ethyl
acetate fraction was the least active at both concentrations. Only the aqueous fraction was active atinhibiting the heat
induced albumin denaturation with a maximum inhibition of 63.91 % at 200 μg/ml.
Conclusion: These findings offer pharmacological support to the suggested folkloric uses of Tetrapleura tetrapteraleaf in the
management of inflammatory conditions, in south-western communities in Nigeria
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