Evaluation of Antioxidant Activity of Thesium viride Hill (Santalaceae)
https://doi.org/10.51412/psnnjp.2022.30
Keywords:
TAC method, antioxidant activity, flavonoids content, ethyl acetate, Thesium virideAbstract
Background: Thesium viride is commonly called 'Huntu' in Hausa. It is a hemi-parasite subshrub from the family Santalaceae. The aerial part of the plant is used in the treatment of jaundice, liver enlargement, splenomegaly and ulcers. Despite its uses in diseases that may be attributed to free radical there was no studies to evaluate of the antioxidant activity. The study aimed to evaluate the antioxidant activity of 70% aqueous ethanol extract of T. viride (AETV) and its fractions; ethyl acetate (ETV), butanol (BTV) and aqueous (ATV).
Methods: The plant was collected at Karaukarau, Giwa Local Government Area of Kaduna State and was identified and authenticated at the Herbarium Unit of the Department of Biological Sciences, Ahmadu Bello University. The aerial part of the plant collected was air dried under the shade and powdered. It was macerated with 70% aqueous ethanol and a portion of the extract was successively fractionated using ethyl acetate, n-butanol and water. The free radical scavenging activity of the 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), reducing power of ferricyanide ion (FRAP), total antioxidant capacity (TAC) of the phosphomolybdenum method and the estimation of total phenolics (TP) and total flavonoids (TF) contents have been determined for all studied extracts/fractions by spectrophotometric methods.
Results: Both TP (155.47 mg gallic acid equivalent [GAE]/g ± 10.32 mg GAE/g) and TF contents (103.29 mg quercetin equivalent [QE]/g ± 4.43 mg QE/g) were determined to be highest in ETV. The DPPH free radical, FRAP assays and TAC showed a concentration dependent activity with ETV exhibited the highest antioxidant capacity as determined by all the methods. Using Pearson's
correlation analysis between the values of FRAP-TAC (R2 = -0.91) at p < 0.05 indicates the viability of the two models for evaluating antioxidants from T. viride.
Conclusion: The antioxidant evaluation based on the models used in this study revealed that AETV and especially the ETV possess antioxidant activity.
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