Preliminary study on the anti-bacterial activity of 2 cultivars of Acalypha wilkesiana on bacterial isolates of clinical significance

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Mercy I. Aboh
Oludolapo S. Katibi
Oluwakanyinsola A. Salawu
Kazeem T. Olatunji
Omolola T. Fatokun
Adeola T. Kola-Mustapha
Peters O. Oladosu


Background: Anti-microbial resistance has become a major problem in clinical practice making it necessary to search for newer molecules. Acalypha wilkesiana, is a herb commonly used traditionally in Nigeria to treat gastrointestinal disorders, wounds and skin infections like impetigo.

Objectives: To determine the in-vitro anti-bacterial activity of crude extracts of 2 cultivars of Acalypha wilkesiana (Macrophylla & Hoffffmanii) 

Methods: In-vitro antibacterial activity was investigated by agar diffffusion and micro-broth dilution techniques. Bacterial isolates tested were Escherichia coli, Methicillin Resistant Staphylococcus aureus, Staphylococcus aureus, Salmonella paratyphi, Pseudomonas aeruginosa and Klebsiella pneumoniae.

Results: The ethanol extract of both cultivars were more active than the ethyl acetate and n-hexane extracts having diameter zones of inhibition against all the bacteria tested at a range of 12.00-22.00 The lowest MIC of the crude ethanol extract was seen with E. coli (1000 µg/ml) and S. aureus (1000 µg/ml; 2000 µg/ml) while the most active fractions (R2, R4, G3 and G4) of the crude ethanol extract of both cultivars had greatest activity against MRSAand P. aeruginosa (156 µg/ml). 

Conclusion: This study shows that Acalypha wilkesiana has good broad spectrum anti-bacterial activity with the potential for development as lead compounds to combat multi-drug resistant organisms like MRSA. 

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Aboh, M. I., Katibi, O. S., Salawu, O. A. ., Olatunji, K. T. ., Fatokun, O. T., Kola-Mustapha, A. T., & Oladosu, P. O. . (2022). Preliminary study on the anti-bacterial activity of 2 cultivars of Acalypha wilkesiana on bacterial isolates of clinical significance: The Nigerian Journal of Pharmacy, 56(2). Retrieved from


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