Hepatoprotective effect of the ethanolic leaf extract of Carissa edulis (vahl) apocynaceae on carbon tetrachloride induced liver toxicity in rats

Authors

Keywords:

Carissa edulis, hepatoprotective, biochemical parameters, hepatocellular

Abstract

Background: There are claims by herbalist and even indigenes of Plateau state on the efficient use of Carissa edulis in the management of liver diseases. This work was aimed at assessing these claims to determine the hepatoprotective effects ofCarissa edulis.

Methods: The ethanolic leaf extract of Carissa edulis was scrutinized for hepatoprotective activity against Carbon tetrachloride (CCl4 ) induced liver damage. Biochemical parameters were studied to assess the hepatoprotective activity of the ethanolic extract in serum. Total Bilirubin, Total Protein, Albumin, Aspartate Transaminase, Alanine Transaminase, Alkaline Phosphatase, Total Cholesterol, Triglyceride and High-Density Lipoprotein were determined to assess the effect of the extract on CCl4 induced hepatic damage. Other test conducted include, liver antioxidant test like glutathione, malondialdehyde, superoxide dismutase, catalase and histopathological assessment of liver sections..

Results: The markers in the animals treated with CCl4 showed elevated concentration indicating severe hepatic damage by CCl4. A significant decrease in serum markers was observed in rats treated with plant extract signifying the effect of the plant extract in restoring normal functional ability of the hepatocytes.

Conclusion: These results imply that the ethanolic leaf extract of Carissa edulis has a protective effect against CCl4 induced hepato-cellular injury.

Author Biographies

Yocy Yohana Izam, Department of Pharmacology,

Faculty of Pharmaceutical Sciences, University of Jos.

John Chinenye Aguiyi, Department of Pharmacology,

Faculty of Pharmaceutical Sciences, University of Jos

References

Althnaian TI, AlbokhadalmEI, Bathr SM (2013). Biochemical and histopathological study in rats intoxicated with carbon tetrachloride and treated with camel milk. Springer Plus 2(1): 57 – 64 https://doi.org/10.1186/2193-1801-2-57

Camp L, Benilde B, Anna RG, Robert JA, Jones, LM, Claudia O, Senthil Kumar, S (2004). (Eds). Reports of the thematic strategy for sill protection volume introduction and executive summary, EUR 2131 EN1.

Khan AA & Alzohairy M (2011). Hepatoprotective effect of Camel milk against CCl4 induced Hepatotoxicity in rats. Asian Journal of Biochemistry, 6(2), 171 – 180. https://doi.org/10.3923/ajb.2011.171.180

Donatus IA, Sardjoko TA, Verneulen NP (1990). Cytotoxic and Cytoprotective activities of Curcumin.

Effects of Paracetamol induced cytotoxicity, lipid per oxidation and glutathione depletion in Rats hepatocytes. Biochemical Pharmacology 39, 1869 – 1875.5.

Li R, Gou N, Nfu Z, Ding G, Zou Y & Wang Z (2011). Hepatoprotective actions of Radix paenial rubra

aqueous extract against CCl4 induced hepatic damage. Molecules, 8684 - 8693. https://doi.org/10.3390/molecules16108684

Kyszejko B &Lyzzywe G (1999). Effect of sub lethal concentration of Deltametrin on biochemical parameters of blood serum of carp (Cyprinus carprol). Acta Ichtyol EF Piscatorial, 292, 109 – 116.

Buyakasik Y, Goker H, Buyakasik NS, Sayinalp N, Hasnedaroglu IC, & Ozebe OI (2008) . Effect of platelet count on secretion capacity, formularization and use of formulae for evaluation of platelets secretion in thrombocytopenic patients. Blood Coagulation Fibrinolysis 19: 633 –637.

Emerole GO, Thabrew M (1983). Changes in some rat hepatic microsomal Component induced by prolonged administration of chloroquine. Biochemical Pharmacology, 32 (20), 3005 –3007. https://doi.org/10.1016/0742-8413(85)90104-5

Sofowara A (1986).The state of medicinal plant research in Nigeria 1st Edition University Press Limited, Ife Nigeria page 145-148.

Jaeschke H, Gores GJ, Cederbaun AI, Hinson JA, Pessayre D & Lemaster JJ (2002). Mechanism of

Hepatotoxicity. Toxicological Sciences. 65(2), 166 – 176.

Dasgupta A, Ramsey KL, Smith JS, Auble DT (2004). Sir antagonist 1 (San 1) is a ubiquitanligage. Journal of Biological Chemistry, 278(26), 26830 –26938. https://doi.org/10.1074/jbc.M400894200

Ciszewski TS, Ralston S, Acheson D, Wasi, S, Strong SJ (1993). Protein level and Plasma-pheresis

Intensity. Transfusion Medicine, 3, 59 – 65. http://doi.org10.1111/j.1365-3148.1993.tb00105.x.

Anthea MH, Jean MC, William J, Susan W, Maryonna Q, Laitart D, Wright JD (1993). Human biology and health (3rdedn). New Jersey: Eaglewood Cliffs Prentice Hall

Fahim FA, Esmat AY, Fadel HM, Hassan KF (1999). Allied studies on the effect of Rosmarinus officinalis (Linn) on experimental Hepatotoxicity and mutagenesis. International Journal of Food Science and Nutrition, 50, 413 - 427. https://doi.org/10.1080/096374899100987

Adil M, Allahverdiyev N, Olga, NO (2013). Fighting Multidrug Resistance with Herbal Extracts, Essential Oils and their component. Science Direct 17(1): 245-259.

Ostapowicz G, Fontana RJ, Scheldt FV, Larson A, Davern TJ, Han SH & Lee WM (2002). Acute liver failure study group results of prospective study of acute liver failure at 17 tertiary care centers in the United States. Annals of Internal Medicine, 137(9), 947 - 954. https://doi.org/10.7326/0003-4819-137-12-

-00007

Alyoussef H, Hassan W (2010). Phytochemical and biological studies of the aerial parts of Carissa edulis growing in Saudi Arabia. Biosciences Biotechnology Research Asia 7(2): 635 - 46.

https://doi.org/10.13005/bpj/314

Nedi T, Mekonnen N & Urqa K (2004). Diuretic Effect of the Crude Extract of Carissa edulis in Rats. Journal of Ethno pharmacology 95: 57-61. https://doi.org/10.1016/j.jep.2004.06.017

Mahheswari M & Rao PGM (2005). Antihepatoprotective effects of Grape seed in Rats. Indian Journal of Pharmacology 37 (3), 179. https://doi.org/10.4103/0253-7613.16216

Frenzel C, Teschke R (2016). Herbal Hepatotoxicity: Clinical Characteristics and Listing Compilation. International Journal of Molecular Sciences. 1(7), 50 - 88. https://doi.org/10.3390/ijms17050588.

Shankar MB, Parikh, JR. Geetha M, Mehta RS, & Saluja, AK (2005). Hepatoprotective activity of benzopyrone from Tephrosia purpunea pers. Journal of Natural Remedies 5(2), 115 –120.

Lorke D (1983). A new approach to acute toxicity testing, Arch Toxicology 54(4) 275-287.

Hicks LE, Langham RA, Takenaka J (1969). Formation on Membrane – bound Polyribosome. American Journal of Public Health. 2(1), 202 – 777.

Canadian Centre for Occupational Health and Safety (2013) what is LD50 and LC50. Retrieved October 8, 2015, from Canadian centre for occupational health and safety website:

http://www.ccohs.ca/oshanswer/chemicals/id50.html.

Hale JM (1983). The action of hydrogen sulfide on polarographic oxygen sensors, Aquatic and physiological applications. Gnaiger E, Forstner H (Eds) Springer, Berlin, Heidelberg, New York; 73 – 75.

Banker GJ, Verma SK (1987). Preliminary studies of flowering and fruiting in Carissa edulis. Journal of

Natural Product 4(7): 1056-1057.

Burkil HM (1985). The useful plant of West Africa, Second Edition Volume/Families. A-D. Royal Botanical Gardens. London: Kew 40(2): 176

Ibrahim H (1997). Pharmacognostic and biological analgesic activity studies of Carissa edulis Vahl. PhD Ahmadu Bello University Zaria, Nigeria. 157-60, 235 - 45, 306-307.

Omino FA& Kokwaro JO (1993). Ethno botanical of Apocynaceae Species in Kenya. Journal of Ethno Pharmacology 4: 167-180.

Mehmetcik G, Ozdemirier G, Kocak-Toker N, Cevikbas U & Uysal M (2008). Effect of Pretreatment with artichoke extract on carbon tetrachloride induced liver injury and oxidative stress. Experimental toxicology pathology, 60(6), 475 – 480.

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Published

2021-09-30

How to Cite

Yohana Izam, Y. ., & Chinenye Aguiyi, J. . (2021). Hepatoprotective effect of the ethanolic leaf extract of Carissa edulis (vahl) apocynaceae on carbon tetrachloride induced liver toxicity in rats. The Nigerian Journal of Pharmacy, 55(2), 15–23 | https://doi.org/10.51412/psnnjp.2021.9. Retrieved from https://psnnjp.org/index.php/home/article/view/87