Haematological And In-Vivo Antioxidant Modulatory Activities Of Justicia Secunda Vahl [Acanthaceae] Leaf Extract In Phenylhydrazine-Induced Anemic Rats.
Keywords:
Justicia secunda, Phenylhydrazine, Anaemia, Anti-anemic activity, Oxidative stress, AntioxidantsAbstract
Background: Medicinal plants represent an important resource in the fight against diseases and, search for new lead compounds in the drug industry. Research into ethnomedicinal claims made about these plants serves as a key starting point in the investigation of their phytotherapeutic potential. The leaf of Justicia secunda is used ethnomedicinally in many climes to treat anaemia.This study was aimed at evaluating the anti-anaemic and /n-vivo antioxidant activities of the methanolleafextract off.secunda(MEJS) in phenylhydrazine-inducedanaemic rats.
Methods: Anaemia was induced in Wistar albino rats by intraperitoneal administration of 60 mg/kg of Phenylhydrazine (PHZ) for two consecutive days.This was followed by treatment with 100, 200 and 400 mg/kg doses of MEJS and 300 mg/kg ferrous sulfate, which served as the standard drug for two weeks. Blood samples were collected on days 0, 7 and 14 post-induction of anaemia and analyzed for changes in haematological parameters and antioxidant activities.
Results: Administration of PHZ led to a significant (p < 0.05) decrease in the number of red blood cell counts, haemoglobin and hematocrit concentration and an elevation of the mean cell volume. PHZ administration equally led to a significant reduction in the activities of Superoxide dismutase (SOD), Catalase (CAT) and Glutathione peroxidase (GPx) enzymes. The extract showed a significant reversal of the anaemic effects induced by PHZ as well as elevated the In- vivo antioxidant activities of SOD, CAT, and GPx. It equally caused a reduction in levels of lipid peroxidation as evidenced by decreased levels of malondialdehyde.
Conclusion: The methanol extract of 1. secunda leaf has the potential to be useful in the management of acute anaemia, at a level comparable to that offerrous sulfate, used in the management of iron deficiency anaemia.
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